Human Anti-Diphtheria Toxin/Toxoid (DTX) IgA ELISA kit

Sample

Serum and other biological fluids

Species Reactivity

Human

Intended Use

The Human Anti-Diphtheria Toxin IgA ELISA Kit detects and quantifies diphtheria toxin (DTX) or toxoid-specific IgA in human serum or plasma of exposed or immunized hosts. This immunoassay is suitable for:
Determining immune status relative to non-immune controls;
Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
Qualifying and/or standardizing vaccine batches and protocols.
This kit is for research use only (RUO), not for diagnosis or therapeutic purposes.

Storage

2-8°C until the expiration date printed on the box label.

Sensitivity

The diphtheria toxin coating level, HRP conjugate concentration and Low NSB Sample Diluent are optimized to differentiate anti- diphtheria toxin/toxoid IgA from background (non-antibody) signal with human serum samples diluted 1:200.

General Description

Diphtheria is a contagious disease of the upper respiratory tract. The illness is characterized by sore throat, low fever, and an adherent membrane (a pseudo membrane) on the tonsils, pharynx, and/or nasal cavity. Diphtheria also causes the progressive deterioration of myelin sheaths in the central and peripheral nervous system leading to degenerating motor control and loss of sensation. Common diphtheria has largely been eradicated in industrialized nations through widespread vaccination, e.g., the DPT (Diphtheria–Pertussis–Tetanus) vaccines are recommended for all school aged. These contain a toxoid of the disease-causing diphtheria toxin.

Monitoring the efficacy of vaccines by determining the anti-toxin levels in patients, including for clinical trials using new formulation of vaccines, is often required. The Creative Diagnostics Anti- Diphtheria ELISAs will quantify antibodies produced by vaccines, or from exposure to the toxin-producing organisms.