Inquiry Basket
Loading ......
The Human 17-ketosteroids (17-KS) ELISA Kit is a specialized in vitro diagnostic and research tool designed for the accurate, sensitive, and specific quantification of 17-ketosteroids in human biological samples. It leverages the enzyme-linked immunosorbent assay (ELISA) technology, combining high specificity of antigen-antibody reactions with the efficiency of enzyme catalysis. This kit is widely applied in clinical endocrine research, reproductive medicine, and the study of adrenal gland function, enabling researchers and clinicians to obtain reliable data on androgen metabolism, which is crucial for understanding and evaluating conditions related to endocrine disorders.
17-ketosteroids (17-KS) are a class of steroid compounds produced by the metabolism of androgens in the adrenal cortex, testes, and ovaries. They are primarily excreted in urine and present in serum/plasma, serving as important biomarkers for assessing the functional status of the hypothalamic-pituitary-adrenal (HPA) axis and gonadal function. Abnormal levels of 17-KS are closely associated with various diseases, such as congenital adrenal hyperplasia, polycystic ovary syndrome (PCOS), adrenal tumors, and hypogonadism. Traditional methods for 17-KS detection have limitations like low specificity, radioactive hazards, or complex operations. The Human 17-KS ELISA Kit addresses these drawbacks by adopting a sandwich or competitive ELISA format (depending on design) with highly specific monoclonal antibodies, ensuring accurate quantification while being safe, easy to operate, and suitable for high-throughput sample analysis.
Figure 1. 17-ketosteroids.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended use
Product Features
Three Core Advantages
| Parameter | Details |
| Product name | Human 17-ketosteroids ELISA kit |
| Sample | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
| Intended Use | Human 17-ketosteroids ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the 17-ketosteroids. This ELISA kit is for research use only, not for therapeutic or diagnostic applications. |
| Storage | Store the kit reagents at 2-8°C. For more detailed information, please download the following document on our website. |
Compared to alternative 17-KS detection methods, this ELISA kit stands out for the following reasons:
vs. RIA: Eliminates radioactive reagents (no radiation hazards or waste disposal issues) and has a longer shelf life (12 months vs. 3 – 6 months for RIA reagents).
vs. LC-MS: Requires no expensive mass spectrometry equipment (reducing initial investment by over 80%) and simpler sample pretreatment (no derivatization steps), making it accessible to small and medium-sized laboratories.
Reliable performance
The performance of this reagent kit has been rigorously validated by methodology. The standard curve has a wide range and excellent fit, ensuring the accuracy of quantitative results. Through the addition of recycling experiments, it has been proven that it has good accuracy and precision in urine matrix.
Various Applications
The detection of 17-KS is a key step in exploring diseases related to excessive androgen production in the adrenal gland, such as PCOS and CAH. By using this kit, researchers can conveniently screen specific populations, analyze hormone metabolism characteristics
Important Role in Pharmacology
In the field of drug development, this kit can be used to evaluate the effects of new drugs on steroid hormone synthesis and metabolic pathways. In toxicology, it is one of the effective indicators for evaluating whether compounds have endocrine disrupting effects
The Human 17-ketosteroids ELISA Kit is a high-performance tool for accurate quantification of androgen metabolites. With its high sensitivity, specificity, user-friendly operation, and broad application scope, it addresses the limitations of traditional detection methods and meets the needs of both research and clinical auxiliary scenarios. Whether for studying endocrine physiology, screening endocrine disorders, or monitoring drug efficacy, this kit provides reliable, efficient, and cost-effective solutions, making it an ideal choice for laboratories and clinical institutions focused on androgen metabolism research.
A1: Because the excretion rate of 17-KS fluctuates throughout the day, collecting 24-hour urine can eliminate the influence of this circadian rhythm and obtain its total excretion, thus more accurately reflecting the overall functional status of the adrenal gland and gonads.
A2: Yes, it's crucial. 17-KS in urine mainly exists in the form of water-soluble sulfate or glucuronic acid esters, which must be dissociated into free steroid forms through acid hydrolysis in order to be effectively recognized by antibodies in ELISA kits.
A3: It's not possible. This kit is designed to detect the level of total 17 ketosteroids and provides a comprehensive indicator. To determine individual components such as DHEA and androsterone separately, techniques such as chromatography-mass spectrometry are required.
A4: This kit is specifically optimized for urine samples. The concentration and form of 17-KS in serum are different from those in urine, and direct use may result in inaccurate detection. It is not recommended to use serum samples.
Loading ......
