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Creative Diagnostics provides you with highly specialized SARS-CoV-2 Pseudovirus Neutralization Assay service solutions to support you in accurate and reliable assessment of neutralizing antibody responses targeting SARS-CoV-2. Our platforms are supported by the latest technologies and bioanalytical method validation to provide highly sensitive, reproducible and stable neutralization assays. These services support a range of applications including but not limited to vaccine development and research, characterizing therapeutic antibodies and development of targeted antiviral drugs. Through neutralization measurement, we can support you in optimization of vaccine formulation, accurate assessment of protective immunity and faster development of effective solutions in preclinical and clinical stages.
Promega GloMax® Discover
Molecular Devices ImageXpress Pico
Beckman Coulter CytoFLEX
Neutralization Assay which is used to perform an in-depth analysis of the neutralization potential of antibodies in a biological system. With years of experience in bioanalytical method development, sensitive detection methods, and assay optimization, we provide a fully customizable pseudovirus-based solution to meet your research requirements. Our advanced platforms are designed for accurate detection and quantification of neutralization potential, offering high sensitivity, specificity, and reproducibility. We help with profiling immune responses, evaluating the effectiveness of vaccines and therapeutic antibodies, and assessing the potency of antibodies against SARS-CoV-2 variants, with the goal of helping you to accelerate vaccine development and antibody discovery and to develop effective treatment strategies. We provide:
To meet the need for accurate and reliable evaluation of neutralizing antibody activity, we provide comprehensive SARS-CoV-2 Pseudovirus Neutralization Assay services. Utilizing advanced bioanalytical platforms and highly sensitive detection technologies, we achieve precise measurement and characterization of antibody-mediated viral neutralization. We offer standardized sample processing and extensive experimental validation with multiple readouts (luminescence based infection inhibition assays, fluorescence imaging, ELISA-based quantification) to guarantee robust sensitivity, specificity and biological relevance of all generated data. This platform is commonly employed for evaluation of vaccine efficacy, development of therapeutic antibodies and monitoring of immune protection. Our stringent quality control and broad technical experience guarantees high reproducibility, stability and data consistency on a per-project basis. Our core services include:
Production of SARS-CoV-2 pseudoviruses pseudotyped with full-length or variant-specific spike protein and validation of their infectivity and stability.
Design of the neutralization assay according to the type of samples, the desired readout platform, and the specific research question.
Measurement of neutralizing activity using a luminescence-based reporter assay, fluorescence imaging, or ELISA-based readouts.
Neutralization against multiple SARS-CoV-2 variants, including emerging variants.
Processing of serum/plasma or purified antibodies under standardized conditions.
Calculation of IC50/ID50 values, IC50/ID50 inhibition curves, and antibody potency comparisons.
Inclusion of proper controls and repeats of experiments to determine assay stability and reproducibility.
Prepare a complete report, containing assay set-up and description, raw data, statistical analysis, and final data interpretation requested for the project.
Understand research objectives and design tailored assays.
Prepare samples and select appropriate assay methods.
Perform antibody neutralization experiments using a pseudovirus system.
Perform data analysis and generate detailed reports.
Provide project summaries and subsequent technical support.
SARS-CoV-2 Pseudovirus Neutralization Curve. The plot shows the percentage of viral infection inhibition at different serum dilutions. IC50 values can be derived from the dose-response curve to evaluate antibody potency. X-axis: Serum dilution (fold, log2 scale). Y-axis: Neutralization (%) from 0 to 100%.
Absolutely! We use a pseudovirus that can not replicate but mimic the entry process of SARS-CoV-2. It is not infectious with the actual virus.
Yes. We can test the neutralizing activity of serum, plasma, or purified antibodies and it is ideal for vaccine efficacy and immune monitoring studies.
Yes! We have multiple SARS-CoV-2 variants in our system and you can test and compare the neutralizing protective effects of the antibodies against different variants.
Yes, we will provide IC50/ID50 values and a detailed report that visually represents the neutralizing potency of the antibodies.
Absolutely reliable. We strictly control experimental conditions to ensure high sensitivity, reproducibility, and data stability.
To use our SARS-CoV-2 pseudovirus neutralization assay services, please contact Creative Diagnostics, your expert immunology service provider. From research-scale SARS-CoV-2 pseudovirus neutralization assay services to scale-up, our team is ready to support your R&D innovation.
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