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Oxytocin (Oxt) is a neuropeptide hormone synthesized by the hypothalamus and released by the posterior pituitary gland. Accurately quantifying oxytocin levels is crucial for a deeper understanding of its broad roles in the central nervous system and peripheral system. This Oxytocin Enzyme Immunoassay (EIA) kit utilizes advanced competitive principles to provide researchers with a sensitive, specific, and easy-to-use tool for accurately detecting oxytocin concentrations in samples such as plasma, serum, cerebrospinal fluid, and tissue homogenates, contributing to cutting-edge explorations in neuroscience, psychology, endocrinology, and animal behavior.
In neuroendocrine research, understanding oxytocin's dynamic changes in response to external stimuli or pathological conditions is essential for unraveling the mechanisms underlying social behavior disorders, mood disorders, and reproductive health issues. However, the accurate detection of oxytocin has long been challenging due to its low physiological concentration in biological fluids and potential cross-reactivity with structurally similar peptides. Traditional detection methods, such as radioimmunoassay (RIA), often suffer from drawbacks like radioactive waste handling, short reagent shelf life, and limited sensitivity. The Highly Sensitive Oxytocin ELISA Kit overcomes these limitations by leveraging highly specific monoclonal antibodies and a sandwich ELISA format, enabling sensitive, safe, and user-friendly oxytocin quantification—filling a critical gap in modern neuroendocrine and behavioral research.
Figure 1. Oxytocin.
The reagent kit is based on mature enzyme immunoassay technology. The core principle is the competition method: endogenous oxytocin in the sample competes with pre added enzyme labeled oxytocin (tracer) to bind to specific anti oxytocin antibodies coated in the microplate. After incubation and washing to remove unbound components, the substrate solution is added, and its color intensity is inversely proportional to the concentration of oxytocin in the sample. Accurate quantification of oxytocin content in unknown samples can be achieved by measuring absorbance using an enzyme-linked immunosorbent assay (ELISA) reader and comparing it with a standard curve. This reagent kit has been carefully optimized and the process is efficient, and can complete the detection within about 4 hours.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended use
Product Features
Three Core Advantages
| Parameter | Details |
| Product name | Oxytocin Enzyme Immunoassay Kit |
| Sample | Plasma, Saliva, Clarified Milk, Tissue Culture Media |
| Intended Use | The Oxytocin Enzyme Immunoassay Kit is designed to quantitatively measure Oxytocin present in serum, plasma, saliva, clarified milk and tissue culture media samples. |
| General Description | The neuropeptides, oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornel Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology and neurotransmitter, recent studies have begun to investigate oxytocin's role in various behaviors, including orgasm, social recognition, pair bonding, anxiety, and maternal behaviors and is important in male reproductive physiology. |
Compared to alternative oxytocin detection methods, the Highly Sensitive Oxytocin ELISA Kit offers distinct advantages:
vs. Radioimmunoassay (RIA): RIA requires radioactive isotopes, which pose safety risks, have short half-lives (requiring frequent reagent replacement), and generate radioactive waste. This ELISA kit is non-radioactive, has a 12-month shelf life, and is safer to use—without compromising sensitivity.
vs. Standard ELISA Kits: Most standard oxytocin ELISA kits have an LOD of > 0.5 pg/mL, which is insufficient for detecting low oxytocin levels in cerebrospinal fluid or stress models. This kit's LOD of < 0.05 pg/mL enables detection of physiological concentrations, expanding research possibilities.
Exceptional Sensitivity for Critical Low-Level Detection
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of progesterone present in the follicular phase of the menstrual cycle and in men. This is essential for obtaining clinically meaningful data across all physiological states.
Streamlined, Time-Efficient Workflow
The competitive ELISA format allows for a rapid and simplified procedure. With pre-coated plates and optimized reagents, the entire assay can be completed in approximately 90 minutes, facilitating timely decision-making in both research and clinical settings, and enabling high-throughput screening.
High Specificity for Accurate Clinical Assessment
The antibody used in this kit exhibits high affinity and specificity for progesterone, minimizing interference from other structurally similar steroid hormones. This ensures the accuracy and reliability of results, which is paramount for fertility assessment and pregnancy monitoring.
The Highly Sensitive Oxytocin Enzyme Immunoassay Kit represents a significant advancement in oxytocin detection technology, addressing the longstanding challenges of low sensitivity, poor specificity, and complex operation in traditional methods. By combining ultra-high sensitivity, broad sample compatibility, rapid workflow, and reliable performance, this kit empowers researchers to explore oxytocin's role in neuroendocrine and behavioral processes with unprecedented precision. Whether investigating the molecular mechanisms of social behavior, evaluating potential oxytocin-based therapeutics, or studying reproductive physiology, this kit provides a robust, cost-effective, and user-friendly tool.
A1: No. The kit is for research use only (RUO) and not validated for clinical diagnostic purposes. It can be used for human research samples (e.g., from healthy volunteers or research participants in approved studies) but not for patient diagnosis or treatment monitoring.
A2: The standard oxytocin solution (prepared by diluting the provided standard with assay buffer) is stable for 24 hours when stored at 2-8°C. For best results, prepare fresh standard solutions before each assay.
A3: No. The monoclonal antibodies target the intact oxytocin peptide (amino acid sequence: CYIQNCPLG). They do not cross-react with oxytocin metabolites (e.g., oxytocin fragments) or other related neuropeptides, ensuring detection of only active oxytocin.
A4: The kit is validated for human, rat, mouse, and non-human primate samples. While oxytocin has high sequence homology across mammals, we recommend performing a pilot experiment with a small number of samples from other species (e.g., dog, pig) to confirm compatibility.
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