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The High-Sensitivity Testosterone ELISA Kit is a cutting-edge diagnostic and research tool specifically designed for the accurate quantification of testosterone levels in biological samples. As a critical hormone regulating male reproductive function, secondary sexual characteristics, and metabolic processes, testosterone plays a pivotal role in andrology and endocrinology research. This kit addresses the growing demand for precise, reliable, and efficient testosterone detection, enabling researchers to explore hormone-related disorders (such as hypogonadism, infertility, and polycystic ovary syndrome) and physiological mechanisms with enhanced sensitivity and specificity. It is widely adopted in academic laboratories, pharmaceutical R&D centers, and clinical research institutions, serving as a cornerstone for advancing studies in reproductive health, endocrine balance, and related disease pathogenesis.
Testosterone is the primary androgen steroid hormone, playing a critical role in the development of male reproductive tissues, promoting secondary sexual characteristics, regulating libido, maintaining muscle mass and bone density, and influencing overall health in both men and women. Accurate measurement of testosterone levels is vital for diagnosing and managing conditions such as hypogonadism, polycystic ovary syndrome (PCOS), and certain cancers, as well as for monitoring hormone replacement therapy (HRT) and assessing endocrine status in athletic and aging populations. Traditional testosterone detection methods, such as radioimmunoassay (RIA), often suffer from limitations including radioactive hazard risks, narrow detection ranges, and long assay times.
Figure 1. Testosterone.
This ELISA kit is based on the competitive principle. Endogenous testosterone in the sample competes with a testosterone-enzyme conjugate (horseradish peroxidase, HRP) for binding sites on a limited amount of testosterone-specific antibody that is pre-coated onto the microplate. After an incubation period, the plate is washed to remove unbound materials. A substrate solution is then added, producing a colorimetric signal. The intensity of this signal is inversely proportional to the concentration of testosterone in the sample, allowing for accurate quantification.
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
Intended Use
Key Features of the Product
Performance
| Parameter | Details |
| Product name | Testosterone ELISA Kit |
| Sample | Saliva |
| Intended Use | This Kit is an Enzyme Immunoassay for the quantitative measurement of free active testosterone in saliva. |
| General Description | Testosterone (17β-hydroxy-4-androstene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the β position at C-17. This steroid hormone has a molecular weight of 288.4 daltons. Testosterone is the most important androgen secreted into the blood. In males, primarily the Leydig cells of the testis secrete testosterone; in females approximately 50% of circulating testosterone is derived from peripheral conversion of androstenedione, approximately 25% from the ovary and 25% from the adrenal glands. |
| Performance Characteristics |  |
Compared to alternative testosterone detection methods and conventional ELISA kits, this high-sensitivity kit stands out for the following reasons:
High Sensitivity for Low Concentration Detection
This kit is engineered for high sensitivity, capable of accurately quantifying the low levels of testosterone found in women, children, and hypogonadal men. This is critical for obtaining meaningful data across the full spectrum of physiological conditions.
Streamlined, Time-Efficient Workflow
The competitive ELISA format allows for a shorter total assay time compared to traditional methods. With pre-coated plates and optimized reagents, the entire procedure can be completed in under two hours, significantly enhancing laboratory throughput and efficiency for large-scale studies.
High Specificity in Complex Matrices
TThe antibody used in this kit exhibits high affinity and specificity for testosterone, minimizing interference from other structurally similar steroid hormones present in serum or saliva. This ensures the accuracy and reliability of results in complex biological samples.
The High-Sensitivity Testosterone ELISA Kit is an indispensable tool for andrology and endocrinology research, addressing the critical need for accurate, sensitive, and efficient testosterone detection. Its ultra-high sensitivity, broad sample compatibility, and cost-effectiveness make it superior to traditional methods (such as RIA) and standard ELISA kits, enabling researchers to explore subtle hormonal changes, study disease mechanisms, and advance pharmaceutical R&D. Whether used in basic science laboratories investigating testosterone's physiological roles or in clinical research centers studying hormone-related disorders, this kit delivers consistent, reliable results that drive scientific progress. With comprehensive technical support and strict quality control, it provides researchers with the confidence to tackle complex endocrine and reproductive health challenges, making it a top choice for modern andrology and endocrinology research.
A1: No. This kit is strictly for research use only (RUO) and is not validated or approved for clinical diagnosis, patient monitoring, or therapeutic decision-making.
A2: The kit requires a standard microplate reader capable of measuring absorbance at 450 nm (with a reference wavelength of 630 nm). No specialized equipment (such as radioactive counters or LC-MS/MS systems) is needed, making it compatible with most laboratory setups.
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