High Sensitivity Kanamycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in biological samples (cell lysate, urine, serum, recombinant protein, and fermentation liquid, etc.).
Contents of Kit
No.
Components
Size
Storage Conditions
1
Kanamycin-coated Plate
1 × 96-well Plate
2-8°C
2
Negative control
1.0 mL
2-8°C
3
Kanamycin Standards
6 × 1.0 mL
2-8°C
4
Kanamycin Ab#1
6.0 mL
2-8°C
5
HRP-Conjugated Ab#2
6.0 mL
2-8°C
6
10×Sample diluent
15 mL
2-8°C
7
20× Wash Solution
28 mL
2-8°C
8
Stop Buffer
12 mL
2-8°C
9
TMB Substrate
12mL
2-8°C
Storage
Store the kit at 2-8°C. The shelf life is 12 months when the kit is properly stored.
Kanamycin residue in the production of biological products may lead to abnormal reactions of human beings, thus strict MRLs have been established. This kit is a rapid test product for the determination of kanamycin residues which is sensitive, accurate and time-saving. It can considerably reduce the operation errors in the assay.
Reconstitution And Storage
IMPORTANT: All reagents should be brought up to room temperature before use (1–2 hours at 20–25°C/ 68–77°F). Solutions should be prepared just prior to ELISA test. All reagents should be mixed by gently inverting or swirling prior to use. Prepare volumes that are needed for the number of wells being run. Do not return the reagents to the original stock tubes/bottles. Using disposable reservoirs when handling reagents can minimize the risk of contamination and is recommended. 1.Preparation of 1X Wash Solution Mix 1 volume of the 20X Wash Solution with 19 volumes of distilled water.
Citations
Publication ()
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Background
Kanamycin is a broad-spectrum antibiotic that belongs to the class of aminoglycosides. It is derived from the bacterium Streptomyces kanamyceticus and is commonly used in the treatment of bacterial infections. As an antibiotic, kanamycin works by inhibiting bacterial protein synthesis. It binds to the bacterial ribosomes, which are responsible for protein production, and disrupts the translation process. This interference prevents the bacteria from synthesizing essential proteins, leading to inhibition of bacterial growth and eventual cell death.
Figure 1. Mechanism of action of Kanamycin and amikacin and the associated drug resistance. (Source: Sheikh, B. A. et al., 2021)
Kanamycin is widely used in biomedicine, treatment of animal diseases, and in the food industry. However, as kanamycin is not metabolized in the body, residues of kanamycin in biologics pose a serious hazard to humans, causing ototoxicity, nephrotoxicity, and neurotoxicity to varying degrees. Therefore, the use of kanamycin in biological products and animal food and its residue detection have been regulated in many countries. This High Sensitivity Kanamycin ELISA Test Kit (DEIA-WZ048V) provides a rapid, sensitive, and reproducible method for the determination of kanamycin residues in samples, and is suitable for the detection of kanamycin residues in gene and cell therapy plasmid release and related biological products.
Alternative Names
Kanamycin Kanamycin ELISA Kanamycin ELISA Kit KAM ELISA KAM ELISA Kit KNM ELISA KNM ELISA Kit KA ELISA KA ELISA Kit
References
1. Sheikh B A, et al. Development of new therapeutics to meet the current challenge of drug resistant tuberculosis. Current Pharmaceutical Biotechnology. 2021, 22(4): 480-500.
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References
Monoclonal antibody-based enzyme-linked immunosorbent assay for the aminoglycoside antibiotic kanamycin in foodstuffs
Monoclonal antibodies to the aminoglycoside antibiotic kanamycin (KM) were obtained as a result of the complex immunization of mice with glutaraldehyde conjugates of BSA with KM, tobramycin (TM), and gentamicin. With the use of these antibodies, an indirect competitive enzyme-linked immunosorbent assay was developed. This method allows for the determination of up to 1.2 ng/ml of an antibiotic in water solutions, milk, and eggs, and up to 2.5 ng/ml in honey. The recovery rate in these products spiked with KM was 83, 84, and 96%, respectively. The assays of KM based on homologous and heterologous solid-phase conjugates were estimated. Under these conditions the cross-reactivity with TM could vary from 7 to 54%. The same indexes for amikacin were more constant and reached 7–8%. The other aminoglycosides showed no inhibitory activity.
Kanamycin is an antibiotic of Streptomyces discovered by Umezawa and others, and was purified as its sulfate, C18H36O11N4•H2S04•H2O. The potency of this antibiotic was decided to be expressed by the weight of its base. Takeuchi and others studied the toxicity of this antibiotic and reported that it was low in acute toxicity to mice, rats and rabbits, and in chronic toxicity to mice and dogs. This antibiotic is water-soluble and basic, therefore, further detailed studies on chronic toxicity, especially to nervous system, were required before the clinical studies. The present paper describes studies on chronic toxicity to dogs and cats and on ototoxicity to rats.
COA
Publication 
Figure 1. Mechanism of action of Kanamycin and amikacin and the associated drug resistance.
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