The Human ACTH (Corticotropin) ELISA Kit is a robust and sensitive tool designed for the quantitative detection of Adrenocorticotropic Hormone (ACTH) in human serum, plasma, and cell culture supernatants. Utilizing an advanced enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers with a reliable method to obtain precise data crucial for studying the hypothalamic-pituitary-adrenal (HPA) axis. Its optimized protocol ensures high specificity and reproducibility, making it an indispensable asset for research in endocrinology, stress physiology, and related metabolic disorders.
Adrenocorticotropic Hormone (ACTH) is a pivotal peptide hormone produced and secreted by the anterior pituitary gland. It plays a central role in regulating cortisol production from the adrenal cortex, thereby influencing stress response, metabolism, and immune function. Accurate measurement of ACTH levels is essential for understanding various physiological and pathological conditions, including Cushing's syndrome, Addison's disease, and chronic stress responses.
Figure 1. Adrenocorticotropic Hormone.
his ELISA kit employs a sophisticated sandwich immunoassay principle, which ensures high specificity and sensitivity. The microplate is pre-coated with an antibody specific to human ACTH. When samples or standards are added, ACTH antigens bind to these immobilized antibodies. Subsequent steps involve the addition of a biotinylated detection antibody and enzyme-conjugated streptavidin, leading to a colorimetric reaction whose intensity is directly proportional to the concentration of ACTH in the sample. This design minimizes non-specific binding and enhances the accuracy of the results.
Figure 2. Structure of Adrenocorticotropic Hormone.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications and Performance
Performance
Intended Use
Key Features of the Product
| Parameter | Details |
| Product name | Human ACTH(Corticotropin) ELISA Kit |
| Sample | Serum, plasma, tissue homogenates and other biological fluids. |
| Intended Use | ACTH ELISA Kit allows for the in vitro quantitative determination of ACTH concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Standard Curve |  |
User-Friendly Workflow
The kit's optimized protocol minimizes complexity and hands-on time: pre-coated plates eliminate the need for antibody coating, and pre-diluted reagents reduce preparation steps.
High Specificity
The antibodies used in this kit have been meticulously selected for high specificity to human ACTH, showing minimal cross-reactivity with related hormones. This ensures that the measurements accurately.
Precision
This kit is engineered for high sensitivity, capable of detecting even low physiological levels of ACTH with a detection limit as low as 5.0 pg/mL . The excellent precision, with both intra- and inter-assay coefficients of variation (CV) typically less than 10%.
The Human ACTH (Corticotropin) ELISA Kit is a reliable, high-performance tool for quantifying human ACTH in research settings. By combining ultra-sensitivity, species-specificity, and a user-friendly workflow, it addresses the core challenges of HPA axis research—from detecting low physiological ACTH levels to avoiding cross-reactivity with other hormones. Whether investigating circadian rhythm, endocrine disease mechanisms, or the efficacy of HPA-targeted therapeutics, this kit delivers accurate, reproducible results that support robust scientific conclusions. Its flexible format and broad sample compatibility further make it a valuable asset for both basic research labs and pharmaceutical R&D teams.
A1: After receiving the reagent kit, it should be stored at a temperature of 2-8 ° C. Under these conditions, all ingredients remain stable until the expiration date printed on the packaging box, typically 6 to 12 months. Avoid freezing reagents.
A2: No. This kit is specifically designed for detecting human adrenocorticotropic hormone. Due to the potential species-specific variations of ACTH protein, cross reactivity with samples from other species such as rodents and primates cannot be guaranteed. If intended for such use, user validation should be conducted.
A3: This kit uses a sandwich ELISA format, which is usually more specific and sensitive, making it an ideal choice for detecting intact proteins such as ACTH. Some other test kits may use competitive formats, which are typically suitable for detecting small molecules. Sanming treatment usually provides a wider dynamic range and is less susceptible to the effects of sample matrix.
A4: Yes, Creative Diagnostics provides comprehensive technical support for all of our products. If you have any questions about the testing plan, troubleshooting, or data interpretation, please contact our technical team.
