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The Human Growth Hormone Releasing Factor (GHRF) ELISA Kit from Creative Diagnostics is a sophisticated and reliable tool designed for the quantitative detection of Growth Hormone Releasing Factor (also known as Growth Hormone-Releasing Hormone, GHRH) in human serum, plasma, and other biological fluids. Utilizing a sensitive enzyme-linked immunosorbent assay (ELISA) format, this kit provides researchers with a robust method to obtain precise data crucial for studying the regulation of growth hormone secretion and its implications in various physiological and pathological conditions. Its optimized protocol ensures high specificity and reproducibility, making it an indispensable asset for research in endocrinology, neurobiology, and growth disorders.
Growth Hormone Releasing Factor (GHRF) is a key neuropeptide produced by the hypothalamus. It plays a central role in stimulating the synthesis and release of growth hormone (GH) from the anterior pituitary gland. Growth hormone, in turn, is essential for linear growth, metabolism, and cell repair throughout life. Accurate measurement of GHRF levels is vital for understanding various physiological processes and pathological conditions, including growth disorders (e.g., dwarfism, gigantism), pituitary diseases, and metabolic syndromes.
Figure 1. Growth Hormone.
This ELISA kit employs a sandwich immunoassay principle. The microplate is pre-coated with an antibody specific to human GHRF. When samples or standards are added, GHRF antigens bind to these immobilized antibodies. Subsequent steps involve the addition of a biotinylated detection antibody and enzyme-conjugated streptavidin, leading to a colorimetric reaction whose intensity is directly proportional to the concentration of GHRF in the sample. This design minimizes non-specific binding and enhances the accuracy of the results.
Sample Compatibility
Sensitivity Range
Main Uses
Key Features of the Product
Performance
Intended Use
Applications in Research
| Parameter | Details |
| Product name | Human Growth Hormone Releasing Factor ELISA Kit |
| Sample | blood, plasma |
| Intended Use | This Enzyme Immunoassay kit is designed to measure a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. |
| Storage | Store the kit at 2-4°C upon receipt. For more detailed information, please download the following document on our website. |
Simplified Sample Processing
Unlike competing kits that require complex sample preprocessing (e.g., solid-phase extraction for hypothalamic tissue), this kit allows direct use of homogenates, serum, or plasma after simple centrifugation.
Unmatched Sensitivity for Low-Abundance Samples
Hypothalamic tissue homogenates and pediatric plasma typically contain GHRF at ultra-low concentrations (1–10 pg/mL), which traditional ELISA kits (sensitivity cannot detect reliably.
High Specificity for Human GHRF
The antibodies used in this kit have been selected for high specificity to human GHRF, showing minimal cross-reactivity with related molecules. This ensures accurate measurement of GHRF concentration in complex sample
The High-Sensitivity GHRF ELISA Kit is an indispensable tool for neuroendocrine and growth research, addressing the critical need for accurate, ultra-sensitive quantification of GHRF. Its unmatched sensitivity for low-abundance samples, simplified processing, cost-effectiveness, and eco-friendly design make it ideal for a wide range of applications—from exploring hypothalamic-pituitary axis function to validating GHRF as a biomarker for growth and metabolic disorders. By overcoming the limitations of traditional methods and competing kits, this kit empowers researchers to advance understanding of GHRF-related pathologies and accelerate the development of targeted therapies—solidifying its role as a must-have asset for neuroendocrine, growth, and metabolic research laboratories.
A1: No. This kit is specifically designed for human GHRF, as its monoclonal antibodies target amino acid sequences unique to human GHRF. For non-human GHRF detection, please select species-specific kits (e.g., Mouse High-Sensitivity GHRF ELISA Kit, Rat High-Sensitivity GHRF ELISA Kit) offered by the manufacturer.
A2: The kit should be stored at 2-4°C upon receipt. Under these conditions, all components remain stable until the expiration date printed on the box, which is typically 6 months. Some components may require storage at -20°C for long-term preservation; please refer to the specific instructions provided with the kit
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