Creative Diagnostics offers Cellular Biosensors for the study of Ligand-Receptor Interactions which enable quantitative and kinetic real-time analysis of ligand binding and cell responses. Combining state of the art detection with stable and robust cell models, the biosensor systems are extremely sensitive, reproducible and stable. Applications of these systems are drug screening, signal transduction and receptor/ligand characterization, target validation, and high throughput discovery in drug and biomed discovery.
Biacore™ T200
Octet® QK
LigandTracer
Creative Diagnostics offers Cellular Biosensors for Ligand-Receptor Interaction services. We have years of experience in cellular assay development, biosensor engineering, and detection technologies to support your study of molecular binding and cell signaling events. We can engineer a custom cellular biosensor platform to fit your research needs, whether it be real-time monitoring of interactions, binding affinity measurements, or signal transduction analyses. Our biosensor platforms are designed with optimal cell models, sensitive detection methods, and robust data analysis tools to ensure specificity, reproducibility, and biological relevance. We provide:
To meet the research and development demands for precise and reliable analysis of ligand-receptor interactions, we provide comprehensive Cellular Biosensors for Ligand-Receptor Interaction services. Employing a wide variety of cellular assay platforms, together with extremely sensitive detection systems, we facilitate reliable real-time measurements of binding and cellular responses. We provide optimized biosensor design and cell model selection, strict validation of assays, and numerous analytical tools (fluorescence resonance energy transfer (FRET), surface plasmon resonance (SPR), high-content imaging, label-free technologies etc.) for excellent sensitivity, specificity and biological relevance. This approach is broadly applicable in drug discovery, receptor pharmacology, signal transduction and drug development. Our extensive quality control procedures and vast experience in the technical knowledge assure each project of high reproducibility, stability and data consistency. Our core services include:
Design genes, construct expression systems, and produce chimeric receptors and ligands.
Establish reporter cells to detect ligand-receptor binding.
Co-culture cells to monitor signal activation in real time.
Ensure cell stability and accurate and reliable signals.
Analyze data, provide reports, and provide technical support.
In the case study using the developed cellular biosensor to evaluate PD-1/PD-L1 immune checkpoint blockade, flow cytometry was used to assess the dose-response relationship between blocking antibody activation of the PD-1/PD-L1 interaction. The percentage of PD-1/PD-L1 interaction activation decreased significantly with increasing blocking antibody concentration, indicating that the antibody inhibited the interaction in a concentration-dependent manner. Data points represent the mean, and error bars represent the standard error. The horizontal axis is a logarithmic scale of antibody concentration.
We use cell lines stably expressing chimeric receptors, combined with rigorous control experiments, to ensure specific and reliable signals and avoid false positives.
Of course! Our platform supports high-throughput screening, enabling rapid and accurate identification of effective ligands or inhibitors, significantly reducing R&D time.
Yes, our sensor design is highly sensitive, enabling us to capture low-affinity but biologically important interactions.
Yes! We first expose the blocking agent to the stimulated cells and then observe changes in signal intensity, allowing us to accurately measure blocking ability.
Of course. We have an experienced team that can design and build custom sensors tailored to your specific receptor or ligand needs.
To use our cellular biosensors for ligand-receptor interaction study services, please contact Creative Diagnostics, your expert immunology service provider. From research-scale cellular biosensors for ligand-receptor interaction study services to scale-up, our team is ready to support your R&D innovation.
