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Creative Diagnostics for rapid production of high quality, high affinity antibodies for sensitive, real-time detection of target molecules in a wide variety of research applications. The repeated immunization at multiple anatomic sites (RIMS) approach, which involves the repeated administration of an antigen at more than one anatomical site, is utilized to increase the breadth of the immune activation, diversity of the B cell repertoires and strength of the resulting antibody response. This approach combined with our high throughput screening and characterization methods, culminates in low background, high specificity and consistent performing antibodies. From the selection of antigens and design of the immunization schedule to the creation of hybridomas and functional validation, each stage of the process is refined to ensure the highest quantity and quality of antibodies.
Harvard Apparatus PHD Ultra™ Microinfusion Pump
Tecan Freedom EVO Liquid Handling System
BD FACSymphony Flow Cytometer
Creative Diagnostics provides Repetitive Immunizations at Multiple Sites Services to help you to more quickly and efficiently develop high affinity and high specificity antibodies for research, diagnostics and therapeutic applications. We have been developing custom antibody solutions for clients for decades. Our expertise in immunology and custom antibody production allows us to offer a complete and customized immunization strategy optimized to the specific antigen and application. Our platform utilizes the strategy of multiple site, repeated antigen delivery to boost immune responses, improve epitope coverage and increase antibody yield. This service can be relied on to generate strong, reproducible antibodies which are suitable for more demanding downstream workflows such as ELISA, Western blotting, immunohistochemistry, and biosensor development. We are dedicated to providing reliable, high-quality antibodies with outstanding performance and specificity. We provide:
To meet the growing demand for high-affinity and highly specific antibodies in research and diagnostic applications, we specialize in providing Repetitive Immunizations at Multiple Sites Services. Carefully designed antigen delivery protocols and strategic multi-site immunization schedules are used to increase immune responses, induce broad epitope recognition and generate high titers. In addition to advanced antigen design, the program also includes careful adjuvant selection and extensive post-immunization validation to support various applications such as screening of therapeutic candidates, validation of biomarkers and establishment of immunoassays. Operated under a comprehensive quality management system and supported by significant technical experience, the program will ensure that all generated antibodies have the highest possible specificity, stability and reproducibility between batches. Our core services include:
We provide tailored antigen design services, including peptide synthesis, recombinant protein expression, and conjugation to carrier proteins. This ensures optimal immune recognition, especially for weakly immunogenic or structurally complex targets.
By administering antigens at multiple anatomical sites over repeated intervals, we stimulate multiple lymphoid regions, enhancing B cell diversity, antibody affinity maturation, and overall immune response strength.
Choose and design adjuvants to match your antigen class and vaccination objective. Our formulations are engineered to drive strong immune activation while reducing tolerance or off-target responses.
We tailor vaccination schedules to the antigen, species, and desired antibody attributes. Booster vaccinations are optimally spaced to enhance memory B cell expansion and antibody quality.
We regularly collect and analyze serum samples to assess antibody titers, binding affinity, and immune kinetics. This data guides real-time optimization of the immunization strategy.
Using state-of-the-art fusion techniques or B cell sorting, we isolate and screen clones producing high-affinity, target-specific antibodies, ensuring successful monoclonal development.
We offer both monoclonal and polyclonal antibody production based on the immunization outcome, with scalable options from small research batches to large preclinical quantities.
Our downstream processing includes affinity purification, isotype verification, endotoxin testing, and concentration adjustment. Each batch undergoes strict quality control to ensure high purity, reproducibility, and application-specific performance.
Analyze antigen properties and immune response goals.
Customize multi-site injection plans with optimal dosage and adjuvants.
Perform repeated immunizations and track antibody titer levels.
Select high-affinity antibodies and confirm specificity.
Scale up antibody production and provide technical assistance.
Comparison of ELISA antibody titer curves. The curves show the absorbance (OD400) values of antibodies produced by repeated multi-site immunization (RIMS, blue dot line) and the control group (red square line) at different serum dilutions. The results showed that the antibody titer of the RIMS group at each dilution was significantly higher than that of the control group, indicating that this immunization strategy can significantly enhance the level of antibody production.
No. We will scientifically arrange the immunization schedule to improve the immunization effect while ensuring the health of the animals. Usually, the immunization cycle is equivalent to or even faster than the traditional method to produce high-titer antibodies.
Of course. We support a variety of animal options such as mice, rats, rabbits, etc., and the injection site will also be optimized according to the animal species, antigen characteristics and experimental purpose.
We provide serum or spleen cells (for monoclonal antibody preparation), and optional ELISA test reports, titer analysis, antibody purification services, and even subsequent hybridoma construction and large-scale production support, all in one stop.
To use our repetitive immunizations at multiple sites services, please contact Creative Diagnostics, your expert immunology service provider. From research-scale repetitive immunizations at multiple sites services to industrial scale-up, our team is ready to support your R&D innovation.
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