Anti-Hev b5 (Major latex allergen) monoclonal antibody (CABT-L2749)

Mouse Anti-Hev b5 (Major latex allergen) monoclonal antibody for ELISA(Det)

Specifications


Host Species
Mouse
Antibody Isotype
IgG2b
Clone
22
Species Reactivity
HEV
Immunogen
Hev b5-MBP fusion protein expressed and purified from E. coli.
Conjugate
Unconjugated

Applications


Application Notes
Recommended working dilution:
ELISA: 1:64 000
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Target


Alternative Names
HEV; Hepatitis E virus
UniProt ID

Citations


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References


Quantitative analysis of fuel-saving potential for waste heat recovery system integrated with hybrid electric vehicle

INTERNATIONAL JOURNAL OF ENERGY RESEARCH

Authors: Gao, Yan; Wang, Xuan; Tian, Hua; Cai, Jinwen; Shu, Gequn

Hybrid electric vehicles (HEVs) with low fuel consumption, low emissions, and long driving range are the ideal transition models between conventional fuel vehicles and pure electric vehicles. The growing demand for increased vehicle efficiency has motivated the introduction of waste heat recovery (WHR) technology in the automotive industry, with the organic Rankine cycle (ORC) as the most promising measure for recycling waste energy. Currently, only a few studies have been conducted to couple HEV and WHR systems. These studies have mainly focused on the hybrid powertrain control strategy, but lack quantitative methods to comprehensively analyze the fuel-saving potential due to the WHR system. In this study, an HEV-WHR integrated system that includes a mechanism-based dynamic model of ORC and a hybrid diesel-electric truck model is established. Further, a quantitative evaluation method that simultaneously considers the negative integrated effects (increased vehicle weight and increased exhaust back pressure) and the positive impact values of the engine, motor, and WHR system on the fuel-saving potential is proposed. Finally, the influence of two environmental factors (wind speed and ambient temperature) on the fuel-saving performance is analyzed. The results reveal that under the standard highway driving cycle (HWY), the negative integrated effects reduce the ideal fuel-saving potential of the HEV-WHR system from 6.10% to 5.42%. However, the optimized performances of the engine, motor, and WHR system improve the fuel-saving rate by 0.39%, 1.81%, and 3.22%, respectively. The results also indicate that the fuel-saving potential increases from 1.62% to 8.60% with increasing wind speed and decreases from 6.70% to 4.25% with increasing ambient temperature.

DEVELOPMENT OF ELISA TEST KITS FOR SEMI-QUANTITATIVE DETECTION OF ANTI-HEV ANTIBODIES IN PIGS IN THE REPUBLIC OF BELARUS

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF BELARUS-AGRARIAN SERIES

Authors: Krasochko, Petr A.; Zhavoronok, Sergey, V; Barysavets, Dzmitry S.; Krasochko, Pavel P.; Alatortseva, Galina, I; Prokopenkova, Tatsiana M.; Davydov, Vladimir V.; Anisko, Ludmila A.; Luhverchik, Ludmila N.; Zhuravleva, Ekaterina S.; Buchukuri, Djemal, V; Zverev, Vitali V.

Viral hepatitis E infection affects up to 80-100 % of domestic pigs worldwide and is characterized by high seroprevalence among domestic pigs in temperate climate countries. Epizootic monitoring of HEV infection is insufficient in the Republic of Belarus due to lack of the required number of available and inexpensive diagnostic ELISA kits with good sensitivity and specificity. In this regard, research on development of domestic ELISA kit for semi-quantitative detection of antibodies to hepatitis E virus in pigs with subsequent assessment of seroprevalence to I IEV in pig population in the Republic of Belarus is relevant. The results of studies on development of enzyme-linked immunosorbent assay kit for semi-quantitative determination of class G immunoglobulins to HEV in pig blood serum using recombinant proteins, including immunodominant amino acid sequences corresponding to the ORF2 and ORF3 proteins of HEV genotype 3 are presented in the paper. The optimal concentration for sorption of ORF2 and ORF3 proteins has been determined, which is 2 mu g/ml and 1 mu g/ml, respectively. The diagnostic sensitivity of this test kit makes 94.8 %, and the diagnostic specificity makes 100 %. Coefficients of variation being the criterion for assessing the intra-serial and inter-serial reproducibility of this test kit, make 3.5 % and 12.4 %, respectively, which allows to obtain reproducible results and identify specific anti-HEV antibodies in all positive samples of pig blood serum. When studying 1235 pig sera samples from various pig farms of Brest, Vitebsk, Gomel, Grodno, Minsk and Mogilev regions, seroprevalence of anti-HEV antibodies has been determined in 168 or 13.6 % of animals. The described diagnostic method can be widely used in science and practice for the further study of seroprevalence of anti-HEV.

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