Hepatitis B Virus Surface Antigen L-protein

Hepatitis B virus (HBV) is a DNA virus that attacks the liver and has three surface antigens: L-protein, M-protein, and S-protein. The L-protein consists of the S-region, Pre-S2 region, and Pre-S1 region, and contains either 389 or 400 amino acids. The N-terminal end of the L-protein is the Pre-S1 region, which binds to a receptor on the surface of hepatocytes, thus facilitating viral entry. In addition, the L-protein plays an important role in the assembly and secretion of the virus.

The three S proteins (L-HBs, M-HBs and S-HBs) differ in their N- terminus but share a common S domain with 4 putative TM domains on their C-terminus [image:Hepatitis-B-Virus-Surface-Antigen-L-protein-1.jpg;alt:structural features] Fig. 1 The structure of HBV envelope proteins.
(Wu S L, et al. Frontiers in Immunology. 2022)

Applications

Study of Infection Mechanisms
Receptor binding studies: Studying the Pre-S1 region of the L-protein can lead to a better understanding of how HBV enters the host cell.
Viral entry process simulation: Using L-protein-derived bio-nanocapsules (BNCs), it is possible to mimic the early infection mechanisms of HBV and study the process of cell attachment and entry into hepatocytes.
Diagnostic Markers
L-proteins and their components (e.g., Pre-S1 and Pre-S2 regions) can be used as markers for diagnosing HBV infection. The structure of L-proteins is specific to HBV infection, which can help doctors diagnose and monitor the disease more accurately.
Vaccine Development
More effective vaccines can be designed to prevent HBV infection by utilizing the immunogenicity of L-proteins.
Antiviral Therapy
Antibodies or peptides specific to the Pre-S1 region of the L-protein can be used to develop antiviral drugs to prevent the virus from entering host cells.

Creative Diagnostics offers a wide range of high-quality Hepatitis B virus Surface Antigen L-proteins of genotypes A to D for research applications. These antigens are rigorously validated for use in HBV mimetics, standard antigens for ELISA, Western blotting, and other techniques. With excellent specificity and sensitivity, these products are reliable tools for developing diagnostic methods and for research use.

The purity of recombinant HBsAg L-protein (genotype A) >95%, determined by SDS-PAGE [image:Hepatitis-B-Virus-Surface-Antigen-L-protein-2.jpg;alt:>95%]

Recombinant HBsAg L-protein (genotype A) Cat. No #DAG-WT3520

The purity of recombinant HBsAg L-protein (genotype B) >95%, determined by SDS-PAGE [image:Hepatitis-B-Virus-Surface-Antigen-L-protein-3.jpg;alt:>95%]

Recombinant HBsAg L-protein (genotype B) Cat. No #DAG-WT3521

The purity of recombinant HBsAg L-protein (genotype C) >95%, determined by SDS-PAGE [image:Hepatitis-B-Virus-Surface-Antigen-L-protein-4.jpg;alt:>95%]

Recombinant HBsAg L-protein (genotype C) Cat. No #DAGC047

The purity of recombinant HBsAg L-protein (genotype D) >95%, determined by SDS-PAGE [image:Hepatitis-B-Virus-Surface-Antigen-L-protein-5.jpg;alt:>95%]

Recombinant HBsAg L-protein (genotype D) Cat. No #DAG-WT3522

Fig. 2 SDS-PAGE images of recombinant HBsAg L-protein (under reducing condition).

Reference

Wu, S., et al. (2022). Immune mechanisms underlying hepatitis b surface antigen seroclearance in chronic hepatitis b patients with viral coinfection. Frontiers in Immunology, 13, 893512.

Cat. No.	Product Name	Expression System
DAG-WT3520	Recombinant Hepatitis B virus Surface Antigen L-protein (genotype A)	Yeast	Inquiry
DAG-WT3521	Recombinant Hepatitis B virus Surface Antigen L-protein (genotype B)	Yeast	Inquiry
DAGC047	Recombinant Hepatitis B virus Surface Antigen L-protein (genotype C)	Yeast	Inquiry
DAG-WT3522	Recombinant Hepatitis B virus Surface Antigen L-protein (genotype D)	Yeast	Inquiry