HCV Core Antigen ELISA Kit (DEIA-Z00189)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Intended Use
The HCV Core Antigen ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the HCV core protein.
Contents of Kit
1. Anti-HCVcAg Antibody Coated Plate
2. FITC-Conjugated Anti-HCVcAg Monoclonal Antibody
3. HRP-Conjugated Anti-FITC Monoclonal Antibody
4. Assay Diluent
5. Triton X-100 Solution
6. 10X Wash Buffer
7. Substrate Solution
8. Stop Solution
9. Recombinant HCVcAg Standard
Upon receiving, aliquot and store recombinant HCVcAg Standard at -20°C and avoid freeze/thaw. Store all other components at 4°C until their expiration dates. For more detailed information, please download the following document on our website.


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Synthesis, biological evaluation and in silico modeling of novel pangenotypic NS5A inhibitors


Authors: Ivashchenko, Andrey A.; Ivanenkov, Yan A.; Aladinskiy, Vladimir A.; Karapetian, Ruben N.; Koryakova, Angela G.; Ryakhovskiy, Alexey A.; Mitkin, Oleg D.; Kravchenko, Dmitry, V; Savchuk, Nikolai P.; Zagribelnyy, Bogdan A.; Ivashchenko, Alexander, V

A series of novel small-molecule pan-genotypic hepatitis C virus (HCV) NS5A inhibitors with picomolar activity containing 2-[(2S)-pyrrolidin-2-yl]-5-[4-(4-{2-[(2S)-pyrrolidin-2-yl]-1H-imidazol-5-yl}buta-1,3-diyn-1-yl) phenyl]-1H-imidazole core was designed based on molecular modeling study and SAR analysis. The constructed in silico model and docking study provide a deep insight into the binding mode of this type of NS5A inhibitors. Based on the predicted binding interface we have prioritized the most crucial diversity points responsible for improving antiviral activity. The synthesized molecules were tested in a cell-based assay, and compound 1.12 showed an EC50 value in the range of 2.9-34 pM against six genotypes of NS5A HCV, including gT3a, and demonstrated favorable pharmacokinetic profile in rats. This lead compound can be considered as an attractive candidate for further clinical evaluation.

Ribosome Pausing at Inefficient Codons at the End of the Replicase Coding Region Is Important for Hepatitis C Virus Genome Replication


Authors: Gerresheim, Gesche K.; Hess, Carolin S.; Shalamova, Lyudmila A.; Fricke, Markus; Marz, Manja; Andreev, Dmitri E.; Shatsky, Ivan N.; Niepmann, Michael

Hepatitis C virus (HCV) infects liver cells and often causes chronic infection, also leading to liver cirrhosis and cancer. In the cytoplasm, the viral structural and non-structural (NS) proteins are directly translated from the plus strand HCV RNA genome. The viral proteins NS3 to NS5B proteins constitute the replication complex that is required for RNA genome replication via a minus strand antigenome. The most C-terminal protein in the genome is the NS5B replicase, which needs to initiate antigenome RNA synthesis at the very 3 '-end of the plus strand. Using ribosome profiling of cells replicating full-length infectious HCV genomes, we uncovered that ribosomes accumulate at the HCV stop codon and about 30 nucleotides upstream of it. This pausing is due to the presence of conserved rare, inefficient Wobble codons upstream of the termination site. Synonymous substitution of these inefficient codons to efficient codons has negative consequences for viral RNA replication but not for viral protein synthesis. This pausing may allow the enzymatically active replicase core to find its genuine RNA template incis, while the protein is still held in place by being stuck with its C-terminus in the exit tunnel of the paused ribosome.

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