HIV type 2 Glycoprotein 36 (aa 390 - 703), recombinant protein from E. coli
Recombinant HIV-ΙΙ gp36
Immunoreactive with all sera of HIV-II infected individuals.
Protein is > 95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
1mg/ml, 20mM PBS pH 7.8, NaCl 0.5M, DTT 1mM, 8M urea and 0.4M imidasole.
2-8°C short term, -20°C long term
HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids. Immunodeficiency develops more slowly with HIV-2.HIV-2 is less infectious in the early stages of the virus than with HIV-1.The infectiousness of HIV-2 increases as the virus progresses.Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion. HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.
HIV1 and HIV2 have similar gag (viral core) and pol (polymerase) regions, they have relatively dissimilar env (envelope) regions. Owing to this lack of homology in the envelope region, there is little serologic cross-reactivity of the antibodies directed
Gp36; HIV 2; Human immunodeficiency virus 2; Human Immunodeficiency Virus Type 2; HIV-2 Gp36; Human Immunodeficiency Virus Type 2 Gp36; Retroviridae; Lentivirus