E. coli GlsA1 [His] (DAG4072)

E. coli GlsA1 (aa 1 - 310) [His], recombinant protein from E. coli Datasheet

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Product Overview
Recombinant E.coli glsA1 (a.a. 1-310), His-tagged
Nature
Recombinant
Tag/Conjugate
His
Purity
> 95% by SDS-PAGE
Format
Liquid. In 20mM Tris-HC buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl 26.1 kDa (231aa) confirmed by MALDI-TOF
Storage
2-8°C short term, -20°C long term
Introduction
Escherichia coli serotype O157:H7 is a Shiga toxin-producing pathogen. This serotype has been reported as an etiological agent in sporadic and outbreak cases of haemorrhagic colitis. It is also associated with haemolytic uraemic syndrome. Certain E. coli serotypes other than O157:H7 also produce Shiga toxin. However, the diarrhoea caused by these other serotypes is not usually bloody. Additionally, E. coli serotype O157:H7 does not ferment sorbitol, whereas most other serotypes do. Therefore, if Sorbitol MacConkey Agar is used as a primary screen, the colonies of E. coli serotype O157:H7 appear colourless (non-sorbitol-fermenting colonies [NSFC]) while colonies of the other serotypes appear characteristically pink (sorbitol-fermenting colonies [SFC]).
Antigen Description
Glutaminase is an amidohydrolase enzyme which generates glutamate from glutamine. Glutaminase has tissue-specific isoenzymes. Importantly, glutaminase is found in glial cells.
Keywords
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia; E. coli; Escherichia coli; Bacillus coli communis

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