Tetracyclines

Tetracyclines are broad-spectrum antibiotics produced by the Streptomyces genus of Actinobacteria whose general usefulness has been reduced with the onset of antibiotic resistance. It exerts a bacteriostatic effect on bacteria by binding reversible to the bacterial 30S ribosomal subunit and blocking incoming aminoacyl tRNA from binding to the ribosome acceptor site. It also binds to some extent to the bacterial 50S ribosomal subunit and may alter the cytoplasmic membrane causing intracellular components to leak from bacterial cells.

In 1948, Chlortetracycline was isolated by Duggan as a metabolite of the actinomyces species Streptomyces aureofaciens. This was the first antibiotic substance of the group of tetracyclines. Currently, there are over 20 tetracyclines available; however, tetracycline, chlortetracycline, oxytetracycline, and doxycycline are the most common ones in veterinary medicine. In addition to therapeutic purposes, in many other countries, tetracyclines are often incorporated into livestock feed at subtherapeutic doses as growth promoters for swine and poultry and in aquaculture. For years, the use of antibiotics as growth promoters has been linked with beneficial aspects (especially increase in nutrient uptake efficiency and commercial revenue for farmers), though there is data that supports the fact that this exercise promotes bacterial resistance, allergic reactions in humans and animals, and changes in environmental microflora and bacterial populations among other detrimental effects.

In all animal species which are used for food production, tetracycline residues (the sum of mother substance and the 4-epimer) are limited in the EU law, commission regulation (EU) No 37/2010 with the following limits (MRLs): 100 ppb in muscle and in milk. CD Tetracyclines ELISA Test Kits are for the quantitative analysis of Tetracycline in milk, honey, meat and other sample types. Creative Diagnostics also provide a full list of antibodies, conjugates for development of Tetracyclines enzyme immunoassay kits.

Key tetracyclines antibiotics analytes in our portfolio:

+ Tetracycline
+ Oxytetracycline
+ Doxycycline