Vitamin B3 (Niacin)

Creative Diagnostics - Food & Feed Analysis

Vitamin B3 (Niacin)

Vitamin B3, also called Niacin, is a water-soluble vitamin that is part of the B vitamin family. Vitamin B3 is the generic term for nicotinic acid, its amide (nicotinamide or niacinamide), and their biologically active derivatives. Low concentrations of this can be produced by the human body from the amino acid tryptophan, however larger concentrations must be ingested via food.

Major sources of Vitamin B3 include:

+ Yeast
+ Meat
+ Fish
+ Dairy products
+ Eggs
+ Green vegetables
+ Cereal grains


The chemical structure of Niacin

Niacin deficiency can cause a condition called pellagra, which causes skin irritation, diarrhea, and dementia. Nutritional status is determined by analysis of plasma or serum concentrations of various niacin metabolites. Since deficiency is rare, the need for analysis of foods is low. Analysis is primarily required to confirm label declarations in fortified foods, feeds or supplements since unfortified foods contain little free niacin.

A number of chemical or microbiological assay techniques have been used for many years. HPLC methods using UV detection are available, although sensitivity and selectivity can be a problem. The use of mass spectrometric (MS) detection following HPLC has been used in more recent methods. Immunoassay method is also available although care is required to select a suitable extraction protocol for different food types.

Creative Diagnostics provide a series of Vitamin B3 antibodies and conjugates for new detection method development.

A list of currently available CD Vitamin B3 products can be downloaded here.

Vitamin B3 (Niacin)

Cat. No Product Name Product Type pdf Inquiry Add to basket
DPATB-H83259 Anti-Vitamin B3 polyclonal antibody Antibody Inquiry
DAGA-122H Vitamin B3 [HRP] Conjugate Inquiry
DAGA-122K Vitamin B3 [KLH] Conjugate Inquiry
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