Custom Small Molecule Antigen Service

Creative Diagnostics - Food & Feed Analysis

Custom Small Molecule Antigen Service

Immunoassay technology (ELISA, LFIA, CLIA and biosensor) in small molecule analysis is increasingly widespread. Small molecule antigens with good immunogenicity are critical for the preparation of antibodies. A stable small molecule antigen with good immunoreactivity for coating or labeling is also very important for the development of test kits. With extensive track record of successful projects in developing small molecule antigens for immunization or coating/labeling, Creative Diagnostics can provide custom small molecule antigen service for your specific needs.

What you can expect from Creative Diagnostics

Creative Diagnostics offers comprehensive services on hapten design and synthesis. We have extensive experience and expertise on:

Select a most suitable position on a drug molecule to attach a linker, which will maximize the specificity, sensitivity and immunogenicity of the immunogen;
Perform total synthesis to make the hapten with a linker when the parent drug molecule has no suitable functional group for conjugation synthesis;
Design and synthesis of the linker with a most suitable length;
Precisely control a most suitable ratio of drug to carrier protein so as to enhance performance of the immunoassay.

The following criteria have governed our structure design for immunizing haptens for polyclonal (PAbs) and/or monoclonal (MAbs) antibody production:

  1. 1. The hapten should be a near perfect mimic of the target structure in size, shape (geometry) and electronic properties.
  2. 2. The chemistry of the target molecule must be understood, reviewed and evaluated for methods of insertion of the attachment handled by established chemistry.
  3. 3. The hapten handle (required for connecting the target-mimic to a carrier protein) should in itself not elicit antibody recognition. Thus, an innocuous methylene handle often is most appropriate, the length of which should be evaluated using one or more homologs.
  4. 4. An appropriate functional group for covalent attachment of the handle to the protein must be compatible with the chemistry of the functional groups on the target.
  5. 5. Several immunizing carrier proteins should be evaluated (here we have found that keyhole limpet hemocyanin (KLH), Limulus polyphemus hemocyanin (LPH), and thyroglobulin conjugates often produce the best PAbs). However, we sometimes also try others selected from conalbumin (CONA), ovalbumin (OVA) and bovine serum albumin (BSA); these may also serve as coating antigen s in the assay development process.

Based on our capacities in antigen design, we’ve completed over 50 projects including a variety of targets:

Amino acid metabolites
Nucleotides
Pesticides residues
Lipids
Small molecule drugs
Chemical contaminants
Saccharids
Toxins
Steroids
Drug residues

Quality Control

Creative Diagnostics provides hapten derivatives and custom complete antigen service according to different requirements from customers. Each product related in the process is strictly identified.

Example of 25-OH Vitamin D3 conjugates identification:

  1. 1. The UV Scan Diagram
       Method: UV scanning spectrum
       Results: A higher absorption value of 25-OHD3-BSA was obtained after the conjugation. The UV diagram of 25-OHD3-BSA containing the characteristic peaks of 25-OHD3 and BSA indicated the successful conjugation.
  2. 2. MALDI-TOF Analysis
       Method: MALDI-TOF-MS
       Results: The Mr of 25-OHD3, BSA and 25-OHD3-BSA were 400.6300, 66430.0000, 71 795.0956, respectively. The calculated coupling ratio was 1 :13.
  3. 3. Immunoassay Analysis
       Method: ELISA
       Results: 25-OH Vitamin D3 conjugates is suitable for the development of an ELISA detection assay.

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