Creative Diagnostics - Food & Feed Analysis
Custom Monoclonal Antibody Against Small Molecules
For some small molecules, it is very difficult to get specific antibody by the traditional antibody production technology. Creative Diagnostics is proud to offer custom rabbit monoclonal antibodies against small molecule haptens. Our state-of-the-art monoclonal antibody screening platform can significantly shorten the screening process and yield high-affinity monoclonal antibodies with broad diversity. CD has successfully developed rabbit monoclonal antibodies using antigen specific B cell cloning technologies. After detection and isolation of antigen specific memory B cells, the antibody variable regions (VH and VL) from single or clonal B cells are cloned and sequenced. Recombinant rabbit monoclonal antibodies are produced, purified, and tested for various applications, such as ELISA, Lateral Flow Immunoassay and CLIA.
Advantages of Rabbit Monoclonal Antibodies Against Small Molecule Haptens
High affinity
Typically, rabbit monoclonal antibodies have 10 – 100 times higher affinity than mouse monoclonal antibodies. On average, the KD values of rabbit monoclonal antibodies range from 10-10 up to 10-12M.
Superior specificity
The high specificity of rabbit monoclonal antibodies against small molecules can significantly improve the assay sensitivity and specificity in food safety immunoassay development.
Small epitope recognition
Partially due to the expression of all three subclasses of CD1 proteins, the nonpeptidic epitope presenting in rabbits is more efficient, rabbit can generate a more vigorous immune response against various small molecule than mice. As a result, rabbits are widely adopted as preferable hosts to generate better antibodies to small molecules and haptens, including vitamins, mycotoxins, drug residues and other chemical contaminants.
B-cell Cloning Technology
High-throughput in vitro B cell culture system and screening technology
Efficient antibody VH/VL gene recombination technology
Efficient mammalian cell expression platform
Multiple antibody detection and validation platform
Summary of Project Proposed
|
Package 1 Customer Antigen to Recombinant Rabbit Monoclonal Antibody |
Package 2 Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody |
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| Service Includes | |||
| Phase 1 | Hapten design & synthesis | ||
| Phase 2 | Immunization of 2 rabbits | ||
| Prebleed | |||
| ELISA titre | |||
| Phase 3 | PBMC collection & antigen specific memory B cells isolation | ||
| B+ cells culture & ELISA screening | |||
| Phase 4 | Cloning and sequencing the antibody variable regions (VH and VL) from antigen specific B cells * | ||
| Transient production of recombinant rabbit monoclonal antibodies in HEK 293 cells | |||
| Antibody Validation | |||
| What You Receive | |||
| 1 | Full project report | ||
| 2 | ELISA data | ||
| 3 | Antibody VH and VL sequences | ||
| 4 | Supernatant from ELISA positive cells (50-80 ul) | ||
| 5 | Purified rabbit monoclonal antibodies (100 ug) | ||
| 6 | Amplified DNA fragments | ||
| 7 | Antigen (1-2 mg) | ||
- Service Includes
-
Phase 1 Hapten design & synthesis :
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 2 Immunization of 2 rabbits :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 2 Prebleed :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 2 ELISA titre :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 3 PBMC collection & antigen specific memory B cells isolation :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 3 B+ cells culture & ELISA screening :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 4 Cloning and sequencing the antibody variable regions (VH and VL) from antigen specific B cells
* :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 4 Transient production of recombinant rabbit monoclonal antibodies in HEK 293 cells :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
Phase 4 Antibody Validation :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody - What You Receive
-
1 Full project report :
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
2 ELISA data :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
3 Antibody VH and VL sequences :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
4 Supernatant from ELISA positive cells (50-80 ul) :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
5 Purified rabbit monoclonal antibodies (100 ug) :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
6 Amplified DNA fragments :
Customer Antigen to Recombinant Rabbit Monoclonal Antibody
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody -
7 Antigen (1-2 mg) :
Hapten Synthesis to Recombinant Rabbit Monoclonal Antibody
*High-throughput next generation sequencing (NGS) or "deep" sequencing of antibody "repertoires" at the single B cell level (B cell repertoire sequencing and B cell repertoire analysis) is also available upon request.
Where do you ship your antigen?
Ship your small molecule conjugate to us at:
Creative Diagnostics
As soon as we start the immunization, we will e-mail you an immunization/production schedule so you’ll know exactly when you can expect your custom rabbit monoclonal antibody.
