Molecular Weight
25.32 kDa
Alternative Names
Foot and mouth disease virus; FMDV; FMDV VP1
Purity
>90% as determined by SDS-PAGE.
Buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
Introduction
FMD is a viral disease affecting cloven-hoofed animals, including domestic and wild bovids. The causative agent is FMDV, a picornavirus. The virus causes a high fever followed by blisters inside the mouth and on the feet that may rupture and cause lameness. FMD has severe implications for animal farming since it is highly infectious and can be spread by infected animals. Of the seven FMD serotypes of this virus, A, C, O, Asia 1 and SAT3 appear to be distinct lineages; SAT 1 and SAT 2 are unresolved clades. FMD serotypes are not uniformly distributed in the regions of the world where the disease still occurs.
The FMDV genome is~ 8,2Kb. It is a small non non-enveloped virus with a pseudo T=3 icosahedral capsid made up of 60 copies of four structural (SP) proteins. P1 of the genome encodes for the capsid proteins (VP1-4) while P2 encodes non-structural proteins (NSP2A/2B/2C) and P3 region (NSP3A/Vpg/3Cpro, and 3D pol). VP1-3 are on the surface, and VP4 is internal. VP1 is the major antigen of FMDV capsid protein that contains the major B-cell epitope, which is the major immunodominant epitope eliciting protective humoral immunity. Upon virus entry into a cell, the viral genome is rapidly translated into a polyprotein which is coand posttranslationally cleaved by viral proteinases into several partially cleaved, likely functional, intermediates and ultimately into 12 mature proteins.
Keywords
Foot and mouth disease virus; FMDV; FMDV VP1
Citations
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