Sample
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
Human Collagen Type VIII Alpha 1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Collagen Type VIII Alpha 1. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Contents of Kit
1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL
Storage
All components of this kit are stable at 2-8°C until the kit's expiration date.
Detection Range
100-2500 pg/mL
Citations
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Gottsch, JD; Zhang, C; et al. Fuchs corneal dystrophy: Aberrant collagen distribution in an L450W mutant of the COL8A2 gene. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 46:4504-4511(2005).
Gerth, J; Cohen, CD; et al. Collagen type VIII expression in human diabetic nephropathy. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION 37:767-773(2007).