Recurrence of SARS-CoV-2 nucleic acid positive test in patients with COVID-19: a report of two cases
BMC PULMONARY MEDICINE
Authors: Wu, Jian; Cheng, Juan; Shi, Xiaowei; Liu, Jun; Huang, Biao; Zhao, Xinguo; Qiu, Yuanwang; Yu, Jiong; Cao, Hongcui; Li, Lanjuan
Background The recurrence of positive SARS-CoV-2 nucleic acid test results in patients with COVID-19 is becoming more important and warrants more attention. Case presentation This study reports 2 cases, a child with mild COVID-19 and an adult female with moderate COVID-19, who were discharged after three consecutive negative nucleic acid tests and were later readmitted to the hospital for recurrence of SARS-CoV-2 nucleic acid positivity. By tracking the patients' symptoms, serum antibodies, and imaging manifestations after readmission, we found that they showed a trend of gradual improvement and recovery throughout treatment. They were cured without additional treatment, with the appearance of antibodies and the recovery of immune functions. Conclusions It is deemed extremely necessary to improve the discharge standard of care. At the same time, nucleic acid detection is recommended to increase the dynamic monitoring of serum antibodies and imaging, strengthen the management of discharged patients, and appropriately extend the home or centralized isolation time.
ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker
BIOCHEMICAL ENGINEERING JOURNAL
Authors: Shabani, Ehsan; Abdekhodaie, Mohammad J.; Mousavi, Seyyed Abbas; Taghipour, Fariborz
A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (R-ct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1-1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 mu A/(decade x cm(2)) with a detection time of only 35 min. Moreover, the results from the developed biosensor were comparable with those measured with a commercial enzyme-linked immunosorbent assay (ELISA) for real samples showing less than 8% difference. The presented MMP-9 biosensor is potentially applicable to the analysis of biological samples, especially in point-of-care (POC) diagnosis or urgent cases analysis, considering its simple fabrication, similar accuracy, shorter detection time, and ease of usage compared with traditional methods such as ELISA.