Feline Immunodeficiency Virus (FIV) ELISA Kit

Sample

Serum

Species Reactivity

Feline

Detection Method

sELISA

Intended Use

Feline FIV ELISA Kit is an Enzyme Immunoassay kit for the qualitative of feline immunodeficiency virus in cat serum.

Contents of Kit

1. Antigen-coated microplate, 8 × 12 strips, 4°C. Unused strips should be sealed tightly in the air-tight pouch.
2. Control A (Negative Control), 2ml (Ready-to-use), 4°C
3. Control B (Cut-off Standard), 3ml (Ready-to-use), 4°C
4. Control C (Positive Control), 2ml (Ready-to-use), 4°C
5. HRP-Streptavidin solution, 20 ml (Ready-to-use), 4°C
6. Sample Diluent Buffer, 100 ml (Ready-to-use), 4°C
7. 20× Wash Buffer, 50 ml, 4°C
8. TMB Substrate, 15 ml (Ready-to-use), 4°C (Protect from light)
9. STOP Solution, 15 ml (Ready-to-use), 4°C
10. Plate sealer, 1 e.a., Room temperature

Storage

Store the unopened kit at 2-8 °C. Use the kit before expiration date.

Precision

The CV values of intra-assay precision is 3.3% and inter-assay precision is 6.7%.

Sensitivity

The diagnostic sensitivity is defined as the probability of the assay of scoring positive in the presence of the specific analytic.
It is >95.5% (95% confidence interval: 84.53% - 99.44%)

General Description

FIV compromises the immune system of cats by infecting many cell types, including CD4+ and CD8+ T lymphocytes, B lymphocytes, and macrophages. FIV can be tolerated well by cats, but can eventually lead to debilitation of the immune system in its feline hosts by the infection and exhaustion of T-helper (CD4+) cells. FIV and HIV are both lentiviruses. However, humans cannot be infected by FIV, nor can cats be infected by HIV. FIV is transmitted primarily through deep bite wounds, where the virus present in the infected cat's saliva enters the body tissues of another cat. FIV+ cats can share water bowls, food bowls (for both wet and dry cat food), and use the same litter box with low danger of transmitting the disease. A vigilant pet owner who treats secondary infections can allow an infected cat to live a reasonably long life. The chance that an FIV-infected cat will pass the virus to other cats within a household is low, unless there is fighting between cats, or wounds present that could allow entry of the virus from infected to non-infected cat.