F4/80 Antibody: Mouse Macrophage and Microglial Marker

Mouse EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1), also known as F4/80, is a cell surface glycoprotein, a member of the EGF TM7 protein family, and 68% of human EMR1 protein amino acid consistency. The expression of F4/80 protein has been found to change significantly during the maturation and activation of macrophages. F4/80 protein is expressed in a variety of mature macrophages, such as Langerhans cells, Kupffer cells, microglial cells, and macrophages located in the peritoneum, intestinal lamina propria, splenic medulla oblongata, lungs, thymus, and bone marrow stroma.

Fig. 1 The antibody to F4/80 showed numerous positive macrophages in liver sinusoids staining green in a patient with alcoholic hepatitis. (Lee J, et al., 2014)

Antibodies to detect F4/80 are useful in a variety of scientific applications, including flow cytometry (FC), immunohistochemistry (IHC), immunocytochemistry, immunomicroscopy, and immunoprecipitation. These antibodies target F4/80 in mouse, human, and rat samples. As the world's leading supplier of macrophage marker antibodies, Creative Diagnostics provides customers with a variety of mouse macrophage surface-specific marker antibodies in various packaging forms, which can be used in various applications such as FC, WB, IF, IHC, etc.

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Applications of F4/80 Antibody

• IHC
  IHC is a technique that enables the visualization of specific antigens in tissue sections. The F4/80 antibody, when used in IHC, allows researchers to precisely identify and locate macrophages and microglial cells within tissue samples. This information is crucial for studying the distribution and activation of these cell populations in various physiological and pathological conditions.
• FC
  Flow cytometry is a powerful method for analyzing and sorting individual cells based on their surface markers. By utilizing the F4/80 antibody in flow cytometry experiments, researchers can easily distinguish macrophages and microglial cells from other cell types in heterogeneous cell populations. This enables the isolation and further characterization of these specific cell populations, providing valuable insights into their functional properties.
• Inflammatory studies
  Macrophages and microglial cells play a crucial role in the immune response and inflammation. The F4/80 antibody serves as an essential tool in investigating the involvement of these cells in inflammatory processes. By targeting the F4/80 antigen, researchers can assess the activation state and polarization of macrophages and microglial cells in various inflammatory models. This information helps in understanding the underlying mechanisms of inflammation and developing targeted therapeutic strategies.

Choose F4/80 antibodies from Creative Diagnostics for your research needs, and unlock new insights into the fascinating world of macrophages and microglial cells. Together, we can unravel the complexities of the immune system and make significant contributions to biomedical research.