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The Ethynylestradiol (EE2) ELISA Kit is a high-performance, user-friendly diagnostic tool designed for the sensitive and specific quantitative detection of ethynylestradiol (EE2) in a wide range of environmental and biological samples. EE2, a synthetic estrogen widely used in oral contraceptives and hormone replacement therapies, is a persistent environmental endocrine-disrupting chemical (EDC) that poses significant risks to aquatic ecosystems and human health even at ultra-low concentrations. This ELISA kit leverages the specificity of antigen-antibody interactions and the sensitivity of enzyme-catalyzed reactions to address the growing demand for rapid, cost-effective, and reliable EE2 detection—overcoming the limitations of traditional analytical methods
17α-Ethynylestradiol (EE2) is a synthetic derivative of the natural hormone estradiol, widely used as a key active component in oral contraceptives and hormone replacement therapy. Due to its widespread use and high resistance to degradation, EE2 has become a pervasive environmental contaminant, often detected in surface waters worldwide. It is recognized as a potent endocrine-disrupting chemical (EDC) capable of inducing adverse effects on aquatic life and wildlife even at very low concentrations (ng/L). Accurate measurement of EE2 is therefore critical for environmental monitoring, risk assessment, and toxicological studies.
Figure 1. Ethynylestradiol (EE2).
Traditional EE2 detection methods (e.g., HPLC-MS, gas chromatography-mass spectrometry, GC-MS) require expensive instrumentation, professional operators, and time-consuming sample pretreatment, making them unsuitable for on-site testing or large-scale sample screening. The EE2 ELISA Kit addresses these challenges by integrating advanced immunological technology: it uses a specific monoclonal antibody against EE2 to bind with EE2 in samples and a horseradish peroxidase (HRP)-labeled secondary antibody to amplify the signal. The intensity of the color reaction (measured by absorbance at 450 nm) is proportional to the EE2 concentration in the sample, enabling rapid and accurate quantitative analysis. This kit fills the gap in rapid, low-cost EE2 detection, supporting both environmental monitoring and biomedical research.
Sample Compatibility
Sensitivity Range
Main Uses
Product Specifications
Intended Use
Key Features of the Product
Performance
| Parameter | Details |
| Product name | Ethynylestradiol (EE2) ELISA kit |
| Sample | Serum, plasma, cell Lysis and other biological samples. |
| Intended Use | For detection of Ethynylestradiol (EE2) in test samples. |
| Storage | Please refer to Reagents and Materials Provided |
Choosing the right EE2 detection tool depends on balancing sensitivity, cost, ease of use, and application needs—and this ELISA kit outperforms alternatives in multiple dimensions:
Exceptional Sensitivity for Trace-Level Detection
This kit is engineered for high sensitivity, capable of detecting EE2 at environmentally relevant concentrations (parts-per-trillion levels). This is paramount for accurate environmental monitoring and for studying low-dose effects in biological systems.
High-Throughput Capability for Large-Scale Screening
The ELISA format is ideal for processing a large number of samples simultaneously in a 96-well plate format. This high-throughput capability makes it significantly more efficient and cost-effective than traditional chromatographic methods (e.g., LC-MS/MS) for screening purposes.
Simple Sample Preparation
For many water samples, minimal pre-treatment (e.g., filtration, pH adjustment) is required before analysis. The straightforward protocol reduces preparation time and minimizes potential errors, making it accessible for laboratories with varying levels of expertise.
The Ethynylestradiol (EE2) ELISA Kit represents a critical advancement in EE2 detection, combining ultra-high sensitivity, excellent specificity, and user-friendly operation to address the needs of environmental monitoring and biomedical research. By overcoming the limitations of traditional methods (high cost, complex operation, long time), this kit enables widespread access to EE2 detection—supporting early warning of environmental pollution, in-depth studies of EE2's biological effects, and efficient large-scale sample screening. With its wide sample compatibility, stable performance, and cost-effectiveness, the kit is not only a practical tool for professional laboratories but also a valuable resource for academic institutions and small-scale monitoring stations. As global concern about endocrine-disrupting chemicals grows, this ELISA kit will play an increasingly important role in protecting ecological health and human well-being.
A1: EE2 is a small molecule (synthetic steroid) with a single antigenic epitope, making it unsuitable for sandwich ELISA formats. The competitive ELISA is the standard and most effective method for accurately quantifying small molecules like EE2.
A2: The antibody used in this kit has been carefully selected for high specificity towards EE2. While it may show some cross-reactivity with structurally similar compounds, this is typically minimal and detailed in the kit's certificate of analysis. For complex samples, sample cleanup may be recommended.
A3: While LC-MS/MS offers excellent specificity and multi-analyte capability, it is expensive, time-consuming, and requires specialized equipment and operators. ELISA provides a highly sensitive, cost-effective, and high-throughput alternative ideal for screening a large number of samples.
A4: Water samples often require filtration to remove particulates and may need pH adjustment to fall within the optimal range for the assay. The kit manual should provide specific sample preparation protocols for different water matrices.
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