Vaccine Grade

Lipopolysaccharides (LPS) (Escherichia coli 0111:B4, vaccine adjuvant)

  • Cat.No.
  • DADF-066
  • Product Name
  • Lipopolysaccharides (LPS) (Escherichia coli 0111:B4, vaccine adjuvant)
  • Subclassification
  • Vaccine grade-PRR Ligands-based adjuvant
  • Classification
  • Adjuvant
  • Storage
  • Shipped at room temperature and it should be stored at 2-8°C. Long term storage at -20°C for upto 2 years. Avoid repeated freeze thaw cycles.
  • Recommended Usage
  • The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more oncentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 °C. For cell culture use, LPS should be reconstituted by adding 1 ml of sterile balanced salt solution or cell culture medium to a vial (1 mg) and swirling gently until the powder dissolves. Solutions can be further diluted to the desired working concentration with additional sterile balanced salt solutions or cell culture media.
  • Description
  • Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs). This product is phenol extracted from E. coli serotype O111:B4. This LPS serotype has been used to stimulate B-cells22 and induce NOS in human hepatocytes. Bacteria with common serotypes have surface antigens (group O, group H, or LPS) which generate the same antibody response.
    Solubility: water (4.9-5.10 mg/ml), cell culture medium (1 mg/ml)
    Turbidity: Slightly hazy
    Prot. Content (%): 0.41
    Potency: 600000 EU/mg
  • Format
  • lyophilized powder; sterile

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