Donkey Sterile Filtered Serum (DAGC285)

Product Overview
The serum was produced from animals in the USA. This serum was collected in USDA approved collection facilities. The animals received inspections under a veterinarian's supervision and were apparently free from infectious and contagious diseases. At no time during collection or processing was the material commingled with any other material of animal origin.
Target
Donkey Serum
Nature
Native
Tag/Conjugate
Unconjugated
Alternative Names
Donkey Serum; Serum
Procedure
None
Purity
0.2 micron (Absolute)
Format
Liquid
Concentration
Hemoglobin: <20 mg/dl
Protein: ≥5.0 g/dl
Size
100ml, 500ml
Buffer
pH: 6.8 - 8.5
Preservative
None
Storage
This serum is stable for ≥ 3 years when stored −20°C ± 5°C. Avoid repeated freezing and thawing.
Keywords
Donkey Serum; Serum

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References


A Comprehensive Appraisal of Laboratory Biochemistry Tests as Major Predictors of COVID-19 Severity

ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE

Authors: Aloisio, Elena; Chibireva, Mariia; Serafini, Ludovica; Pasqualetti, Sara; Falvella, Felicia S.; Dolci, Alberto; Panteghini, Mauro

Context.-A relevant portion of coronavirus disease 2019 (COVID-19) patients develop severe disease with negative outcomes. Several biomarkers have been proposed to predict COVID-19 severity, but no definite interpretative criteria have been established to date for stratifying risk. Objective.-To evaluate 6 serum biomarkers (C-reactive protein, lactate dehydrogenase, D-dimer, albumin, ferritin, and cardiac troponin T) for predicting COVID-19 severity and to define related cutoffs able to aid clinicians in risk stratification of hospitalized patients. Design.-A retrospective study of 427 COVID-19 patients was performed. Patients were divided into groups based on their clinical outcome: nonsurvivors versus survivors and patients admitted to an intensive care unit versus others. Receiver operating characteristic curves and likelihood ratios were employed to define predictive cutoffs for evaluated markers. Results.-Marker concentrations at peak were significantly different between groups for both selected outcomes. At univariate logistic regression analysis, all parameters were significantly associated with higher odds of death and intensive care. At the multivariate analysis, high concentrations of lactate dehydrogenase and low concentrations of albumin in serum remained significantly associated with higher odds of death, whereas only low lactate dehydrogenase activities remained associated with lower odds of intensive care admission. The best cutoffs for death prediction were greater than 731 U/L for lactate dehydrogenase and 18 g/L or lower for albumin, whereas a lactate dehydrogenase activity lower than 425 U/L was associated with a negative likelihood ratio of 0.10 for intensive treatment. Conclusions.-Our study identifies which biochemistry tests represent major predictors of COVID-19 severity and defines the best cutoffs for their use.

The role of CXCR3 and its ligands expression in Brucellar spondylitis

BMC IMMUNOLOGY

Authors: Hu, Xin; Shang, Xiaoqian; Wang, Liang; Fan, Jiahui; Wang, Yue; Lv, Jie; Nazierhan, Shaxika; Wang, Hao; Wang, Jing; Ma, Xiumin

Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-gamma, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-gamma, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-gamma, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-gamma, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-gamma, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-gamma, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-gamma, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.

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