DNase I ELISA Kit

Detection Method

sELISA

Intended Use

This kit employs double-antibody sandwich enzyme-linked immunosorbent assay to detect the content of DNase I.

Contents of Kit

Storage

1. For Box 1, store at -30 to -15°C and transport at ≤ 0°C. For Box 2, store at 2-8°C. Protect the kit from light, and do not freeze it.
2. See the label for the production date and expiration date. The shelf life is 12 months.

Precision

Sensitivity

General Description

Deoxyribonuclease I (usually called DNase I), is an endonuclease of the DNase family coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.
DNase I binds to the cytoskeletal protein actin. It binds actin monomers with very high (sub-nanomolar) affinity and actin polymers with lower affinity. The function of this interaction is unclear. However, since actin-bound DNase I is enzymatically inactive, the DNase-actin complex might be a storage form of DNase I that prevents damage of the genetic information. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner.

Standard Curve