DNase Ⅰ ELISA Kit

Sample

biological fluids

Detection Method

sELISA

Intended Use

For rapid determination of DNase Ⅰ content in samples.

Contents of Kit

1. DNase Ⅰ Coated Plate: 8×12, Ready-to-use;
2. Detection antibody: 150μL×1, 1:100 diluted with Antibody Diluent Buffer;
3. Streptavidin HRP Conjugate: 150μL×1, 1:100 diluted with Enzyme Conjugate Diluent Buffer;
4. DNase Ⅰ Standard: 30μL×1 (200μg/mL), dilute according to the recommended method;
5. Sample Diluent Buffer: 60mL×1, Ready-to-use;
6. Antibody Diluent Buffer: 12mL×1, Ready-to-use;
7. Enzyme Conjugate Diluent Buffer: 12mL×1, Ready-to-use;
8. 20× PBST Wash Buffer: 50mL×1, 1:20 diluted with ddH₂O;
9. TMB Substrate: 11mL×1, Ready-to-use;
10. Stop Solution: 7mL×1, Ready-to-use;
11. Plate Sealer: 5 pieces
12. Datasheet: 1 copy

Storage

Detection antibodies, Enzyme conjugates and Standards should be stored at -20°C, and the other components should be stored at 2-8 °C in the dark. The validity period is 12 months.

Precision

CV%<10%, RE%<±15%

Detection Range

1-64ng/mL

Sensitivity

0.5ng/mL

Standard Curve

It is recommended to use the 4-Parameter fitting method for linear fitting and calculation of this product.
Results of a typical standard operation of a DNase Ⅰ ELISA Kit are listed below. This standard curve was generated at our lab for demonstration purpose only. Users shall obtain standard curve as per experiment by themselves.