E. coli DnaK (DAG3445)

E. coli DnaK, recombinant protein from E. coli Datasheet

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Product Overview
Recombinant Escherichia coli DnaK
Nature
Recombinant
Tag/Conjugate
Unconjugated
Purity
Greater than 95.0% as determined by SDS-PAGE.
Preservative
Sterile filtered colorless solution. The DnaK protein contains 25 mM Tris-HCl buffer (pH 7.5) + 100 mM NaCl + 5 mM DTT and 10% Glycerol.
Storage
2-8°C short term, -20°C long term
Introduction
Escherichia coli serotype O157:H7 is a Shiga toxin-producing pathogen. This serotype has been reported as an etiological agent in sporadic and outbreak cases of haemorrhagic colitis. It is also associated with haemolytic uraemic syndrome. Certain E. coli serotypes other than O157:H7 also produce Shiga toxin. However, the diarrhoea caused by these other serotypes is not usually bloody. Additionally, E. coli serotype O157:H7 does not ferment sorbitol, whereas most other serotypes do. Therefore, if Sorbitol MacConkey Agar is used as a primary screen, the colonies of E. coli serotype O157:H7 appear colourless (non-sorbitol-fermenting colonies [NSFC]) while colonies of the other serotypes appear characteristically pink (sorbitol-fermenting colonies [SFC]).
Antigen Description
Hsp70 (DnaK in E. coli) is perhaps the best characterized small (~ 70 kDa) chaperone. The Hsp70 proteins are aided by Hsp40 proteins (DnaJ in E. coli), which increase the ATP consumption rate and activity of the Hsp70s. It has been noted that increased ex
Keywords
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia; E. coli; Escherichia coli; Bacillus coli communis

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