DNA/RNA Methylation Antibodies

Nowadays, at least 17 and 160 types of chemical modifications have been discovered in DNA and RNA, respectively. Among the known RNA modifications, more than 70 contain methyl groups, so the importance of methylation modification is obvious. Analysis of nucleic acid methylation and their functional effects are critical to epigenetics researchers. Methylation profiles of epigenome are used for disease identification and for research and therapeutic development. Specifically, the detection of methylation in DNA and RNA is essential for the diagnosis and treatment of a wide range of tumor diseases.

Methylation in Cancer

CpG island DNA methylation plays critical roles in X-chromosome inactivation, imprinting and cancers and it is rare in normal cells and increases with age. Evidence shows that the existence of CpG island promoter hypermethylation of tumour suppressor genes such as p16INK4a in melanoma, SET9 in cervical cancer and p15INK4b in hematologic malignancies. In addition, it was also reported that the tumourigenesis of some cancers is caused by the hypermethylation of one copy of a tumour suppressor gene whereas the other copy is naturally mutated or lost, supporting the role of DNA hypermethylation in driving tumourigenesis.

RNA methylation has impact on tissue development, DNA damage repair, as well as tumour initiation and progression through largely regulating RNA stability. The m6A methyltransferase METTL3 is over-expressed in gastric, colon and liver cancers and promotes cancer cell proliferation via suppressing the tumour suppressor SOCS2. The m5C methyltransferase NSUN2 is over-expressed in breast and colorectal malignancies.

Product performance

Fig. 1 Dot blot analysis of 5-Carboxylcytosine pAb (Cat. No DPABB-JX157)	Fig. 2 Dot blot analysis of Am pAb (Cat. No DPABB-JX168)	Fig. 3 Dot blot analysis of 3-mC pAb (Cat. No DPABB-JX159)	Fig. 4 Dot blot analysis of m6A mAb (Cat. No DMABB-JX258)

Fig. 5 ELISA analysis of 5-caC mAb (Cat. No DMABB-JX344)	Fig. 6 IF analysis of DARPP-32 in normal rat ciliary epithelium. (Cat. No CABT-L1171)