Specificity: Der p 7 , a major allergen of Dermatophagoides pteronyssinus (European house dust mite)
Cross-reactivity: Cross-reacts with Der f 7, but with ten-fold stronger reactivity against Der p 7 than Der f 7.
Epitope Sequence: SILDP
Application: Immunoblot
Functional Activity: Inhibits IgE binding to Der p 7, up to 60% inhibition
Multiplex assay ready: PBS only formulation, ready for custom conjugation.
Summary
Specifications
Antibody Isotype
IgE
Clone
WH9
Species Reactivity
Dermatophagoides pteronyssinus
Immunogen
Recombinant Der p 7
Conjugate
Unconjugated
Applications
Application Notes
Suitable for use in ELISA, BL. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
General Notes
Clone WH9 is a monoclonal antibody raised against Der p 7, a major group 7 allergen of the house dust mite Dermatophagoides pteronyssinus. WH9 has been shown to inhibit human IgE binding to Der p 7 by up to 60%, making it a valuable tool for studying allergen‑antibody interactions relevant to allergic disease. The epitope recognized by WH9 on Der p 7 is conformational (loop‑like) and comprises four critical residues: Serine 156, Leucine 158, Aspartic acid 159, and Proline 160. Molecular docking and homology modeling of the Der p 7‑WH9 complex revealed seven hydrogen bonds, two electrostatic interactions, and four hydrophobic interactions between the antibody and the allergen. WH9 exhibits cross‑reactivity with Der f 7 (the homologous allergen from Dermatophagoides farinae), but its reactivity against Der p 7 is approximately ten‑fold stronger than against Der f 7. The antibody was first described by Shen et al. in Clinical & Experimental Allergy (1995;25(5):416‑22). Clone WH9 is widely used in blocking assays, ELISA, and structural studies aimed at designing hypoallergenic vaccines for house dust mite allergy.
Target
Alternative Names
Der p 7; Der p VII; Allergen Der p 7; Dermatophagoides pteronyssinus; House dust mite
Citations
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