Human REDD1 ELISA Kit

Sample

cell lysate

Species Reactivity

Human

Intended Use

This Kit is used for the quantitative measurement of human REDD1 in cell lysate.

Storage

- Upon receipt store all components at 4°C.
- Don't expose reagents to excessive light.

Precision

Intra-assay (Within-Run, n=8) CV=2.2-4.5%
Inter-assay (Run-to-Run, n=4) CV=4.3-7.8%

Sensitivity

The limit of detection (defined as such a concentration of human REDD1 giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: A blank + 3SD blank) is better than 85 pg/mL of sample.

General Description

REDD1 (Regulated in Development and DNA Damage 1) is also called DNA Damage-inducible Transcript 4 Protein, HIF-1 Responsive Protein RTP801 and Dexamethasone-induced gene 2.
REDD1 is rapidly induced by a wide variety of stress conditions; arsenic, hypoxia, dexamethasone, thapsigargin, tunicamycin and heat shock and DNA damages; ionizing radiation, ultraviolet radiation, DNA alkylation. REDD1 is broadly expressed in most adult tissues, with lowest levels in brain, skeletal muscle and intestine and is upregulated in substantia nigra neurons from Parkinson disease patients as well as in alveolar septa of lungs of individuals with advanced emphysema compared with normal lungs.
REDD1 regulates cell growth, proliferation and survival via inhibition of the activity of mTORC by stabilizing the TSC1-TSC2 inhibitory complex and enhances oxidative stress-dependent cell death.
REDD1 inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity.