Crystal violet Assay Kit (Cell viability)

Sample

Adherent cells

Intended Use

For the measurement of cell viability or drug cytotoxicity in adherent cells.

Storage

Store kit at -20°C immediately on receipt and check below for storage for individual components. Kit can be stored for 1 year from receipt, if components have not been reconstituted.
Aliquot components in working volumes before storing at the recommended temperature. Avoid repeated freeze-thaws of reagents.

General Description

Crystal violet Assay Kit (Cell viability) offers an excellent and efficient method for in vitro cytotoxicity studies as well as highthroughput drug screening. It is simple, accurate, reproducible and sensitive. It includes Doxorubicin as a positive control. The Crystal violet staining is directly proportional to the cell biomass and can be measured at 570 nm. This type of staining is a quick and versatile assay for screening cell viability under diverse stimulation or inhibition conditions.

Standard Curve

Data provided for demonstration purposes only

Dose-response curve of HepG2 (Human liver hepatocellular carcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability). Assays were performed according to the kit protocol in triplicate.

Dose-response curve of MCF7 (Human breast adenocarcinoma cell line) cells to Doxorubicin for 72 hours determined by the Crystal violet Assay Kit (Cell viability). Assays were performed according to the kit protocol in triplicate.