Distinguishing host responses, extensive viral dissemination and long-term viral RNA persistence in domestic sheep experimentally infected with Crimean-Congo hemorrhagic fever virus Kosovo Hoti
Applications: ELISA
Reactive species: Sheep
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Abstract: Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) is a tick-borne, biosafety level 4 pathogen that often causes a severe hemorrhagic disease in humans (CCHF) with high case fatality rates. The virus is believed to be maintained in a tick-vertebrate-tick ecological cycle involving numerous wild and domestic animal species, however the biology of CCHFV infection in these animals remains poorly understood. Here, we challenge domestic sheep with CCHFV Kosovo Hoti, a highly pathogenic clinical isolate increasingly utilized in current research. In the absence of prominent clinical signs, the infection leads to an acute viremia and coinciding viral shedding, high fever and markers for potential impairment in liver and kidney functions. A number of host responses distinguish the subclinical infection in sheep versus fatal infection in humans."
Article snippet: Nunc MaxiSorp flat bottom 96-well ELISA plates (***) were coated with 50 ng/well of
recombinant CCHFV Gn protein (Creative Diagnostics,
DAGF-200) or nucleoprotein (Creative Diagnostics, DAGA-3109) in 50 µl 0.06 M carbonate/bicarbonate buffer with pH 9.6 at 4 °C overnight. Coated plates were washed three times using 0.01 M PBS containing 0.05% Tween20 with pH 7.2 (PBS-T).
Figure 1. Antibody responses in CCHFV-infected sheep.
COA
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