Cr (Creatinine) Assay Kit (Colorimetric)

Sample

Serum, Plasma and Urine

Species Reactivity

Universal

Detection Method

ELISA

Intended Use

For quantitative detection of creatinine in serum, plasma and urine.

Contents of Kit

Storage

The kit is stored at 2 - 8°C (Avoid Direct Light), and not be frozen or thawed. The product is valid for 12 months. After opening, store at 2 - 8°C, it can be stable for 30 days, avoid contamination.

Precision

Intra-Assay: CV ≤ 6%
Inter-Assay: CV ≤ 4%
Sample data: Human Serum:60 - 120 μmol/L

Detection Range

25-800μmol/L

Sensitivity

10μmol/L

General Description

Creatinine is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for highenergy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and pcreatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH. Creatinine forms spontaneously from pcreatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.