Mouse Anti-Canine parainfluenza virus monoclonal antibody, clone 8G28

In this investigation a population of dogs at a rehoming center was monitored over a period of 2 years. Despite regular vaccination of incoming dogs against distemper, canine adenovirus type 2 (CAV-2), and canine parainfluenza virus (CPIV), respiratory disease was endemic. Tissue samples from the respiratory tract as well as paired serum samples were collected for analysis. The development of PCR assays for the detection of CPIV, canine adenovirus types 1 and 2, and canine herpesvirus (CHV) is described. Surprisingly, canine adenovirus was not detected in samples from this population, whereas 19.4% of tracheal and 10.4% of lung samples were positive for CPIV and 12.8% of tracheal and 9.6% of lung samples were positive for CHV. As reported previously, a novel canine respiratory coronavirus (CRCoV) was detected in this population (K. Erles, C. Toomey, H. W. Brooks, and J. Brownlie, Virology 310:216-223, 2003). Infections with CRCoV occurred mostly during the first week of a dog's stay at the kennel, whereas CPIV and CHV were detected at later time points. Furthermore, the evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to CPIV and an immunofluorescence assay for detection of antibodies to CHV is described. This study shows that CPIV is present at kennels despite vaccination. In addition, other agents such as CHV and CRCoV may play a role in the pathogenesis of canine respiratory disease, whereas CAV-2 and canine distemper virus were not present in this population, indicating that their prevalence in the United Kingdom is low due to widespread vaccination of dogs.

It is widely acknowledged that rotary positive displacement machines exhibit highly unsteady flow fields that affect their performance. The presence of the operational clearances impacts this unsteady flow field and further affects the performance. However, the exact nature of these unsteady flow mechanisms remains largely unknown that necessitates both detailed experimental investigations and computational modelling. Thus, the present study employs both optical visualization and unsteady Reynolds-Averaged Navier Stokes (URANS) computational modelling methods while focussing on investigating the transient flow field inside a Roots blower, a general type of the rotary positive displacement machine. Straight lobes in a Roots blower provide convenient optical access to experimentally analyse internal flow and compare it with the predictions obtained by standard computational models. In the first part of this paper, this study covers the low-speed experimental investigations using i) High-Speed Camera (HC), ii) the continuous High-Speed Particle Image Velocimetry (CPIV) and, iii) the instantaneous PIV (IPIV) obtained with a double pulse laser and a double shutter camera. Relative merits from these techniques are discussed with respect to the Roots blower unsteady flow mechanisms. In addition, computational analyses are performed using a combination of in-house and commercial modelling methods and the results are compared against the experiments. The results confirm the existence of highly three-dimensional and unsteady flow field where certain distinct flow mechanisms originating from the operational clearances impact the performance of the Roots blower. The study also highlights challenges of the experimental and computational methods used for evaluation of positive displacement machines that impact the accuracy of results. (C) 2019 Elsevier Ltd. All rights reserved.