Product Overview
Empty CPMV VLPs produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression
Tag/Conjugate
Unconjugated
Alternative Names
Cowpea mosaic virus; CPMV
Purity
> 95% , as determined by SDS-PAGE
Format
Lyophilized powder
Concentration
Batch dependent - please inquire should you have specific requirements
Buffer
10 Sodium Phosphate, pH 7.5, 175 mM Trehalose
Introduction
Cowpea mosaic virus (CPMV) is a plant virus of the comovirus group. Infection of a susceptible cowpea leaf causes a "mosaic" pattern in the leaf, and results in high virus yields (1-2 g/kg). Its genome consists of 2 molecules of positive-sense RNA (RNA-1 and RNA-2) which are separately encapsidated. Both RNA1 and RNA2 have a VPg (virus genome-linked protein) at the 5'end, and polyadenylation at the 3' end. Genomic RNA1 and RNA2 are expressed by a polyprotein processing strategy. RNA1 encodes helicase, VPg, protease and RdRp. RNA2 encodes movement protein and coat protein. The virus particles are 28 nm in diameter and contain 60 copies each of a Large (L) and Small (S) coat protein. The structure is well characterised to atomic resolution, and the viral particles are thermostable.
Keywords
Cowpea Mosaic Virus; CPMV; VLP
Citations
Publication ()
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