COVID-19 Spike Protein (RBD) ELISA Kit

Name: COVID-19 Spike Protein (RBD) ELISA Kit
SKU: DEIA-NS2307-28
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

N/A

Detection Method

Sandwich-ELISA

Intended Use

In vitro quantitative determination of 2019-nCoV (S) concentrations in serum, plasma, cell culture supernatant and other biological samples.
Immunogen: 2019 nCoV(S)(RBD) 319 to 541 aa (Variant Alpha/B.1.1.7)
Sequence of spike protein RBD(antigen): TSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSAS FSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGC VIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYF PLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVK

Contents of Kit

No.	Components	Size	Storage Conditions
1	ELISA Microplate(Dismountable)	96T (8×12)	2-8°C/-20°C
2	Lyophilized Standard	2 vials	2-8°C/-20°C
3	Biotin-labeled Antibody	120ul	2-8°C
4	HRP-labeled Conjugate (SABC, 100X)	120ul	2-8°C (Avoid Direct Light)
5	TMB Substrate	10ml	2-8°C (Avoid Direct Light)
6	AssayDilution	5ml	2-8°C (Avoid Direct Light)
7	Sample Dilution Buffer	20ml	2-8°C (Avoid Direct Light)
8	Antibody Dilution Buffer	10ml	2-8°C (Avoid Direct Light)
9	SABC Dilution Buffer	10ml	2-8°C (Avoid Direct Light)
10	Stop Solution	10ml	2-8°C (Avoid Direct Light)
11	Wash Buffer(25X)	30ml	2-8°C (Avoid Direct Light)
12	Plate Sealer	5 pieces

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

Performance Characteristics

Stability: Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.