Cotinine ELISA Kit (DEIA04892)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, urine
Species Reactivity
Human
Intended Use
The Cotinine Direct ELISA Kit is intended for the measurement of Cotinine in serum and urine.
Contents of Kit
1. Microwell coated with polyclonal Ab to Cotinine: 12 x 8 x 1
2. Standard Set (ready to use): 0.5 mL
3. Cotinine HRP Enzyme Conjugate (ready to use): 12 mL
4. TMB Substrate (ready to use): 12 mL
5. Stop Solution (ready to use): 12 mL
Storage
Store the kit at 2-8°C. For more detailed information, please download the following document on our website.
Standard Curve

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References


Elevated plasma cotinine is associated with an increased risk of developing IBD, especially among users of combusted tobacco

PLOS ONE

Authors: Widbom, Lovisa; Schneede, Jorn; Midttun, Oivind; Ueland, Per Magne; Karling, Pontus; Hultdin, Johan

Objective Smoking has previously been associated with inflammatory bowel disease (IBD), but no study has reported on cotinine, an objective, biochemical measure of tobacco use. We aimed at testing the hypothesis that cotinine levels among healthy subjects are associated with an increased risk of developing IBD in later life. Design We analysed plasma cotinine and evaluated corresponding lifestyle questionnaires that included tobacco habits in subjects (n = 96) who later developed late-onset IBD (70 ulcerative colitis (UC) and 26 Crohn's disease (CD)) and in sex and age-matched controls (n = 191). Results Patients who later developed IBD had significantly higher plasma cotinine levels compared to controls. In multivariable analysis, higher log-cotinine was associated with a higher risk of developing IBD (OR 1.34 (95% CI 1.01-1.63)). After stratifying for time to diagnosis, the association was only significant in subjects with shorter time (< 5.1 years) to diagnosis (OR 1.45 (1.09-1.92)). The findings were similar for UC- and CD-cases, but did not reach statistical significance in CD-cases. Although plasma cotinine concentrations were higher in snuff users compared to combusted tobacco users, no increase in the risk of IBD and lower risk of developing IBD among subjects with shorter time (< 5.1 years) to diagnosis was seen among snuff users. Conclusions Cotinine, a biomarker of tobacco use, is associated with increased risk of developing late-onset IBD in general, and UC in particular. No increased risk among snuff users indicates that other components in combusted tobacco than nicotine may be involved in the pathogenesis of IBD among smokers.

Exposure to perfluoroalkyl substances (PFAS) and associations with thyroid parameters in First Nation children and youth from Quebec

ENVIRONMENT INTERNATIONAL

Authors: Caron-Beaudoin, Elyse; Ayotte, Pierre; Sidi, Elhadji Anassour Laouan; McHugh, Nancy Gros-Louis; Lemire, Melanie

Background: Perfluoroalkyl substances (PFASs) are found in several consumer goods. Exposure to PFASs in children has been associated with alteration in thyroid hormones, which have critical roles in brain function. Objective: In 2015, 198 children and youth (3-19y) were recruited as part of the pilot project Jeunes, Environnement et Sante/Youth, Environment and Health (JES!-YEH!), realized in collaboration with four First Nation communities in Quebec. We aimed to evaluate serum concentrations of PFASs in relation to concentrations of thyroid-stimulating hormone (TSH), free thyroxine (T4) and thyroglobulin while adjusting for relevant confounders. Methods: PFASs (PFOS, PFOA, PFHxS, PFNA), 2,2',4,4'-Tetrabromodiphenyl ether (PBDE-47) thyroid parameters (TSH, free T4, and thyroglobulin) were measured in serum samples of 186 participants. Iodine, creatinine, and cotinine were measured in urine samples. Serum levels of PFASs were compared to those measured in the general Canadian population and elsewhere. Multivariate regression analyses were performed to determine associations between PFASs and TSH, free T4 and thyroglobulin. Results: PFOS, PFOA and PFHxS serum concentrations were low. However, PFNA concentrations among participants aged 12 to 19 years old from Anishinabe communities were three times higher than those measured in the Canadian Health Measures Survey (2009-2011) for the same age group (Geometric Means: 3.01 mu g/L and 0.71 mu g/L, respectively) and were particularly higher in the Anishinabe participants aged 6 to 11 years old (GM: 9.44 mu g/L). Few participants had levels of TSH, free T4, and thyroglobulin outside age-specific paediatric ranges. When adjusted for relevant covariates and other contaminants, PFNA serum concentrations were positively associated with free T4 levels (Adjusted beta = 0.36; p = 0.0014), but not with TSH and thyroglobulin levels. No association was observed between the other PFAS and thyroid hormones parameters. Conclusion: This pilot project reveals among the highest exposure to PFNA in children reported until today, and suggests effects of PFNA as an endocrine disruptor, highlighting the importance of investigating the sources and effects of disproportionate exposure to emerging contaminants in some indigenous communities and ban all PFAS at the international scale.

Ali, Badreldin H., et al. "Chronic water-pipe smoke exposure induces injurious effects to reproductive system in male mice." Frontiers in physiology 8 (2017).

Nemmar, Abderrahim, et al. "Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of Flavouring." International Journal of Molecular Sciences 21.4 (2020): 1291.

Ali, Badreldin H., et al. "Ameliorative Effect of Gum Acacia on Hookah Smoke-Induced Testicular Impairment in Mice." Biomolecules 10.5 (2020): 762.

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