Product Name
Complete Freunds Adjuvant (CFA, Vaccine adjuvant)
Subclassification
Adjuvants for protein-based vaccines
Storage
Shipped at room temperature and it should be stored at room temp. DO NOT FREEZE. Stable for 6 months.
Recommended Usage
Suggested Usage: Adsorption of most proteins can be achieved by mixing equal volume of the antigen (preferably in saline) with an equal volume of the adjuvant to form an emulsion. (Ovalbumin/CFA (1:1, v/v was tested). Protein left in the supernatant can be measured by protein assay or specific ELISA. The amount of protein or conjugated peptide used for the primary immunization can be adjusted depending upon availability and immunogenicity of the antigen. Suggested dosage: Mouse/Rat/G.Pig (50-200 ul gel; IM/SC) Rabbit (250 ul; SC/IM).
Description
Complete Freund's Adjuvant (CFA) consists of heat-killed Mycobacterium tuberculosis in a water-in-oil emulsion. Additionally, CFA has ligands for TLR2, TLR4, and TLR9. Injection of antigen in CFA induces a Th1-dominated response. The mycobacterium in Complete Freund′s adjuvant attracts macrophages and other cells to the injection site which enhances the immune response. For this reason, Complete Freund′s Adjuvant is used for initial injections and Incomplete Freund′s Adjuvant for subsequent boosts. Freund′s Adjuvants may be used to produce water-in-oil emulsions of immunogens. Antigens in water-in-oil emulsions stimulate high and long-lasting antibody responses which can be attributed to the slow release of antigen.
Each mL contains 1 mg of Mycobacterium tuberculosis (H37Ra, ATCC 25177), heat killed and dried, 0.85 mL paraffin oil and 0.15 mL mannide monooleate.
pH:6.5
Binding capacity: 1 to 10 µg of endotoxin-free ovalbumin at Ph 7.0. Note: Protein binding will vary with protein concentration, buffer and incubation conditions.
Format
Provided as a ready-to-use ; cell suspension, sterile solution. Clear amber liquid
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