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The Endothelin-2 (ET-2) ELISA Kit is a advanced, rigorously validated diagnostic tool engineered for the accurate, specific, and efficient quantification of human Endothelin-2 (ET-2) in biological samples (e.g., serum, plasma, tissue homogenates, cell culture supernatants). As a key member of the endothelin (ET) family, ET-2 plays pivotal roles in cardiovascular homeostasis (regulating vascular tone, myocardial function) and cancer progression (promoting tumor angiogenesis, cell proliferation, and metastasis). This kit leverages the highly specific sandwich ELISA technology, combining monoclonal antibodies targeting unique epitopes of ET-2, to deliver reliable, reproducible results—empowering researchers to decode ET-2's molecular mechanisms in cardiovascular diseases (e.g., hypertension, heart failure) and cancers (e.g., breast cancer, colorectal cancer), and accelerate translational research and drug development.
Endothelin-2 (ET-2) is a 21-amino-acid peptide closely related to ET-1 and ET-3, but with distinct tissue expression patterns and biological functions. In the cardiovascular system, ET-2 acts as a potent vasoconstrictor (second only to ET-1) and modulates cardiac contractility; dysregulated ET-2 expression is linked to pulmonary arterial hypertension, myocardial fibrosis, and endothelial dysfunction. In cancer, ET-2 is overexpressed in multiple tumor types, where it interacts with endothelin receptors (ETₐ/ETᵦ) to drive tumor microenvironment remodeling, enhance cancer cell invasiveness, and resistance to chemotherapy.
Figure 1. Endothelin-2 (ET-2).
Traditional methods for ET-2 detection (e.g., radioimmunoassay [RIA], immunohistochemistry [IHC]) face critical limitations: RIA carries radioactive hazards and low throughput, while IHC only enables qualitative / (semi-quantitative) analysis. The ET-2 ELISA Kit addresses these gaps by integrating high sensitivity, specificity, and safety—providing precise quantitative data to support both basic research (e.g., ET-2's role in disease pathways) and translational studies (e.g., validating ET-2 as a disease biomarker).
Sample Compatibility
Sensitivity Range
Main Uses
Feature/Specification
Performance
Intended Use
Key Features of the Product
| Parameter | Details |
| Product name | Endothelin-2 ELISA Kit |
| Sample | Human |
| Intended Use | This Enzyme Immunoassay kit is designed to measure a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. |
| Storage | Store the kit at 2-4°C upon receipt. For more detailed information, please download the following document on our website. |
Compared to alternative ET-2 detection methods and competing kits, this ET-2 ELISA Kit stands out for three core reasons:
Adaptability to Diverse Sample Types
Validated for serum, plasma (all common anticoagulants), tissue homogenates, and cell culture supernatants, this kit eliminates the need for custom sample preprocessing (e.g., no additional extraction steps for tissue samples).
High Specificity for Human ET-2 and Broad Applicability
The antibodies used in this kit have been selected for high specificity to human Endothelin-2, demonstrating minimal cross-reactivity with other related substances. This ensures accurate measurement in complex sample.
Ready-to-Use Convenience and Streamlined Workflow
Designed with the researcher in mind, the kit provides pre-coated plates and optimized buffers to significantly reduce preparation time and minimize potential operational errors. The straightforward protocol allows the entire assay to be completed
The Endothelin-2 (ET-2) ELISA Kit is a cutting-edge tool for cardiovascular and cancer research, addressing the critical need for accurate, specific ET-2 quantification. Its ultra-specific detection, rapid workflow, adaptability to diverse samples, and cost-effectiveness make it an ideal choice for both basic research (uncovering ET-2's molecular mechanisms) and translational studies (validating biomarkers, screening drugs). By overcoming the limitations of traditional methods and generic kits, this kit empowers researchers to advance understanding of ET-2-related diseases and accelerate the development of targeted therapies—solidifying its role as a must-have asset for cardiovascular and cancer research laboratories.
A1: No. This kit is specifically designed for human ET-2, as its monoclonal antibodies target amino acid sequences unique to human ET-2. Cross-reactivity with ET-2 from rodents (mouse/rat), rabbits, or other species is < 1%. For non-human ET-2 detection, please select species-specific kits (e.g., Mouse ET-2 ELISA Kit, Rat ET-2 ELISA Kit) offered by the manufacturer.
A2: High lipid content or hemolysis can interfere with antibody binding and absorbance reading. To mitigate this:
For hemolyzed samples: Avoid using samples with visible hemolysis (hemoglobin absorbs at 450 nm, overlapping with TMB's detection wavelength). If hemolysis is unavoidable, dilute the sample 1:2 with sample dilution buffer to reduce interference, and confirm with a spike-and-recovery test to ensure accuracy.
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