Clostridium perfringens and Alpha, Beta and Epsilon toxins ELISA Kit

Sample

Biological samples, Culture supernatants

Species Reactivity

C. perfringens

Detection Method

sELISA

Intended Use

The Elisa Kit can detect the Alpha, Beta and Epsilon toxins of Clostridium perfringens and reveal the multiplicationof the bacterium.
The kit works with culture supernatants as well as biological probe such as liquid intestinal contents and pericardial- or peritoneal fluid.

Contents of Kit

1. Microplate, 2
2. Washing solution (20×) 1×100 mL
3. Colored dilution solution (5×) 1×50 mL
4. Alpha-toxin conjugate(1×-red) 1×6mL
5. Beta-toxin conjugate(1×-yellow) 1×6mL
6. Epsilon-toxin conjugate(1×-blue) 1×6mL
7. Anti-Clos. Perf.conjugate(1×-green) 1×6mL
8. Positive control(1×) 1×4mL
9. Single component TMB(1×) 1×25 mL
10. Stop solution (1×) 1×15 mL

Storage

2-8°C

General Description

Enterotoxaemia is a fatal enteric disease that affects all species of domestic animals and is attributable to a toxigenic type of Clostridium perfringens. The latter is an anaerobic, strongly gram-positive bacterium that has the ability to form heat-resistant endospores. This bacterium is grouped into five types (types A, B, C, D and E) according to the four major lethal toxins, Alpha, Beta, Epsilon, and iota produced. Clostridium perfringens has been shown to be a cause of human diseases such as gas gangrene (clostridial myonecrosis), food poisoning, necrotising enterocolitis of infants, and enteritis necroticans (pigbel). It is also the causative agent of lamb dysentery, ovine enterotoxaemia (struck) and pulpy kidney disease of sheep, and other enterotoxaemic diseases of lambs and calves. Large amounts of toxin in addition to large numbers of Clostridium perfringens cells can usually be detected in the intestinal fluid of the diseased or dead animals.
As Clostridium perfringens is a natural commensal of human and animal intestines, identifying of the bacterium is not enough Toxinotyping and quantifying of the isolated strains are essential.
The Elisa Kit can detect the Alpha, Beta andEpsilon toxins of Clostridium perfringens and reveal the multiplicationof the bacterium.
The kit works with culture supernatants as well as biological probe such as liquid intestinal contents and pericardial- or peritoneal fluid.