Clostridium perfringens and Theta, Beta and Epsilon toxins ELISA Kit

Sample

Biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

ELISA kit for detection of Clostridium perfringens and Theta, Beta and Epsilon toxins

Contents of Kit

1. Microplates: Rows A, C, E, G are sensitised by specific antibodies, rows B, D, F, H by aspecific antibodies.
Row A: anti-Alpha-toxin
Row B: control
Row C: anti-Beta-toxin
Row D: control
Row E: anti-Epsilon-toxin
Row F: control
Row G: anti-Clostridium perfringens
Row H: control
2. Washing solution 1 × 100 ml (20 ×)
3. Colored Dilution buffer 1 × 50 ml (5 ×)
4. Conjugate 4 × 6 ml (1 ×)
5. Control antigen 1 × 4 ml (1 ×)
6. Single component TMB 1 × 25 ml (1 ×)
7. Stopping solution 1 × 15 ml (1 ×)

Storage

2-8 °C

General Description

Enterotoxaemia is a fatal enteric disease that affects all species of domestic animals and is attributable to a toxigenic type of Clostridium perfringens. The latter is an anaerobic, strongly gram-positive bacterium that has the ability to form heat-resistant endospores. This bacterium is grouped into five types (types A, B, C, D and E) according to the four major lethal toxins, Alpha, Beta, Epsilon, and iota produced. Clostridium perfringens has been shown to be a cause of human diseases such as gas gangrene (clostridial myonecrosis), food poisoning, necrotising enterocolitis of infants, and enteritis necroticans (pigbel). It is also the causative agent of lamb dysentery, ovine enterotoxaemia (struck) and pulpy kidney disease of sheep, and other enterotoxaemic diseases of lambs and calves. Large amounts of toxin in addition to large numbers of Clostridium perfringens cells can usually be detected in the intestinal fluid of the diseased or dead animals. As Clostridium perfringens is a natural commensal of human and animal intestines, identifying of the bacterium is not enough. Toxinotyping and quantifying of the isolated strains are essential.
The Elisa Kit can detect the Alpha*, Beta and Epsilon toxins of Clostridium perfringens and reveal the multiplication of the bacterium.
The kit works with culture supernatants as well as biological probes such as liquid intestinal contents and pericardial- or peritoneal fluid.
*Recent mass spectrometry analyses have allowed us to demonstrate that the monoclonal antibody used in this kit is not specific for alpha toxin but for theta toxin and a protease. As theta toxin is almost always associated with alpha toxin, the purpose of this diagnostic kit for enterotoxemia is not affected by this analytical advance. Moreover, theta toxin is less sensitive to the effect of proteases than alpha toxin and is therefore a more sensitive marker of Clostridium perfringens.