Chicken Negative Control Serum (SPF grade)

When blood is taken from a blood vessel and placed in a test tube without the addition of an anticoagulant, the clotting reaction is activated and the blood coagulates rapidly to form a jelly. The clot shrinks and the pale-yellow transparent liquid that precipitates around it is serum, which can also be obtained by centrifugation after coagulation. During the coagulation process, fibrinogen is converted into fibrin clots, so there is no fibrinogen in serum, which is the main difference from plasma. During the clotting reaction, platelets release many substances and various clotting factors change. These components remain in the serum and continue to change, e.g. prothrombin becomes thrombin, and gradually decrease or even disappear if the serum is stored for a long time. During in vitro cell training, its main function is to provide cells with basic nutrients, hormones, growth factors, and binding proteins. These substances can promote cell adhesion and avoid mechanical damage, which plays a certain protective role on cells in culture. Serum is a very complex compound. Although most of its components are known, some are not. In addition, the composition and content of serum often varies with the sex, age, physiological condition and nutritional status of the blood donor animal. Serum is mainly composed of water and various chemical components, including albumin, alpha1, alpha2, beta, gamma-globulin, triglycerides, total cholesterol, alanine aminotransferase, etc. Serum contains various plasma proteins, polypeptides, fats, carbohydrates, growth factors, hormones, inorganic substances, etc. These substances achieve a physiological balance by promoting cell growth or inhibiting growth activity.

Figure 1. Application of chicken Serum.

Chicken Serum is made from healthy chicken blood as the raw material, which is collected, separated and filtered aseptically. It is a clear liquid with no bacteriophage, ultra-low endotoxin, no haemolysis, no foreign matter, no bacteria, fungi, mycoplasma, mycoplasma, chlamydia, etc. It is suitable for laboratory or biochemical research related experiments to meet the various needs of different scientific research experiments. Chicken Serum meets the required quality indicators: 1. Properties, appearance, pale yellow, clear, no haemolysis, no foreign matter, slightly viscous liquid. 2. Protein content 3.5%~5.0% (w/v) 3. Haemoglobin content ≤0.02% (w/v) 4. Sterility test negative 5. Mycoplasma test negative 6. Bacterial endotoxin ≤5 (EU/ml) 7. Negative for bovine diarrhoea virus 8. Escherichia coli phage negative 9. Support cell proliferation (Sp2/0-Ag14) Growth curve (inoculation concentration) Proliferation concentration ≥2.5×10⁶/ml Cell doubling time ≤15h, cloning rate ≥85%. Chicken serum is a natural cell culture medium. It contains abundant nutrients necessary for cell growth and is used for in vitro culture of animal cells. Therefore, chicken serum can also be used for cell culture of hybridoma cells, osteoma cells, primary cells and for the production of vaccines and diagnostic reagents.

Negative controls and positive controls are for "expected results". Any group that can definitely produce the expected results is a positive control, and any group that cannot produce the expected results is a negative control. The blank control is any group without treatment factors is the blank control. Examples of "no processing factors" include using solvents such as physiological saline instead of the solution to be added, or performing surgery on experimental animals without removing certain organs. Negative control and blank control are not necessarily the same thing. The difference between the two is that no processing factors are added to the blank control, while other processing factors are added to the negative control. Chicken negative control serum is a commonly used laboratory reagent and is frequently used in various immunological experiments. This negative control serum is extracted from the blood of healthy chickens. Since these chickens have not been stimulated by immunogens, they do not contain the target antibodies and antigens required for experimental detection. This serum can be used as a negative control experiment in enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), WB and other experiments. The chicken negative control serum can be used in these experiments to establish a standard curve and correct the background signal of the test results, which can greatly Improve the reliability and repeatability of experimental data. Chicken negative control serum is prepared by aseptically collecting blood from healthy chickens to ensure that the blood is not contaminated. The serum is then separated by centrifugation and further purified by gel filtration, ion exchange and other methods. In order to ensure the high purity and consistency of serum control products, negative control serum needs to undergo quality control through multiple testing methods such as PCR testing, ELISA testing, and protein analysis. When used, chicken negative control serum needs to be diluted proportionally as required. Chicken negative control serum serves as a negative control group in immune experiments. By comparing with the experimental group and positive control group, the specificity and reliability of the experimental results can be judged. Chicken negative control serum should be stored at -20°C or lower every day to avoid repeated freezing and thawing to maintain its stability and effectiveness. Pay attention to their quality and storage conditions when selecting and using them to ensure successful experiments.

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