plasma, serum, milk, saliva, CSF, cell culture samples
The CD Human Complement Factor B ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of CFB in human plasma, serum, milk, saliva, CSF, and cell culture samples.
_ Upon arrival, immediately store components of the kit at recommended temperatures up to the expiration date.
_ Store SP Conjugate and Biotinylated Antibody at -20°C.
_ Store Microplate, Diluent Concentrate (10x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C.
_ Unused microplate wells may be returned to the foil pouch with the desiccant packs and resealed. May be stored for up to 30 days in a vacuum desiccator.
_ Diluent (1x) may be stored for up to 30 days at 2-8°C.
_ Store Standard at 2-8°C before reconstituting with Diluent and at -20°C after reconstituting with Diluent.
The minimum detectable dose of human CFB as calculated by 2SD from the mean of a zero standard was established to be 2 ng/ml.
Complement factor B (CFB) is a component of the alternative pathway of complement activation. The zymogen circulates in the blood as a 93 kDa single chain glycoprotein with 739 amino acids. In the presence of C3b, it is cleaved by factor D into a 30 kDa N-terminal non-catalytic Ba fragment and a 63 kDa C-terminal catalytic Bb fragment. The active subunit Bb associates with C3b to form the alternative pathway C3 convertase. Human CFB plays a major role in the initiation of the alternative pathway and in the amplification of C3 cleavage. The polymorphism of CFB influences C3 convertase formation and is associated with age-related macular degeneration.