Human Cleaved Caspase-8 (Asp391) ELISA Kit (DEIA-XY2260)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
cultured cells
Species Reactivity
Human
Intended Use
Human Cleaved Caspase-8 (Asp391) ELISA Kit is an ELISA-based assay using fluorogenic substrates to measure Cleaved Caspase-8 (Asp391) in whole cells.
Contents of Kit
1. Cleaved Caspase-8 (Asp391) Antibody - 1 vial of lyophilized rabbit anti-Cleaved Caspase-8 (Asp391) antibody.
2. Total GAPDH (Ms) Antibody - 1 vial of lyophilized mouse anti-total GAPDH antibody.
3. HRP-conjugated anti-rabbit IgG - 110 μL of HRP-conjugated donkey anti-rabbit IgG secondary antibody.
4. AP-conjugated anti-mouse IgG - 110 μL of AP-conjugated donkey anti-mouse IgG secondary antibody.
5. Substrate F1 Concentrate - 50 μL of a sensitive and stable fluorogenic substrate for horseradish-peroxidase (HRP).
6. F1 Diluent - 10 mL of a solution for diluting the Substrate F1 Concentrate.
7. Substrate F2 - 10 mL of a sensitive and stable fluorogenic substrate for alkaline phosphatase (AP).
8. Blocking Buffer - 35 mL of 10% fetal bovine serum in buffer with preservatives.
9. Wash Buffer (5X) - 60 mL of a buffered surfactant with preservatives.
10. Microplate - 96-well cell culture clear-bottom black microplate and cover for use as a vessel in the assay.
11. Plate Sealers- 4 adhesive strips.
Storage
Store the unopened kit at 2-8°C. Do not use past kit expiration date. This kit contains sufficient materials to run ELISAs on one 96-well plate.

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