Canine Factor VIII Matched Antibody Pair (ABPR-L008)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
Sufficient reagent for 4 x 96 well plates
Sample
Plasma
Species Reactivity
Canine
Intended Use
This antibody pair set comes with matched antibody pair to detect and quantify protein level of Canine Factor VII
Contents of Kit
1. Capture Antibody (yellow): 0.4 ml of polyclonal purified anti-canine FVIII antibody for coating plates.
2. Detecting Antibody (neutral): Four neutral-capped tubes each containing 10 ml of pre-diluted peroxidase conjugated polyclonal anti-canine FVIII antibody for detection of captured cFVIII.
3. Sample Diluent (green): 100 ml bottle containing a green-coloured diluent optimised for dilution of samples.
Note: Reagents are sufficient for at least 4×96 well plates using recommended protocols.
Storage
2-8°C
General Description
Factor VIII is a large glycoprotein (320 kDa) synthesized in the liver. The majority of Factor VIII is cleaved during expression, resulting in a mixture of partially cleaved forms ranging in size from 200-280 kDa. The F.VIII is stabilized in circulation through non-covalent association with von Willebrand Factor. The concentration of F.VIII in normal human plasma is typically 200 ng/mL. In canine plasma, the F.VIII activity is 5-7 fold higher relative to human plasma. F.VIII is a pro-cofactor that is activated through limited proteolysis by thrombin. In this process F.VIIIa dissociates from vWF to combine with activated Factor IX, calcium and a phospholipid surface where it is an essential cofactor in the assembly of the Factor X activator complex. Hemophilia A is a congenital bleeding disorder resulting from an X-chromosome-linked deficiency of F.VIII, occurring with a frequency of 1 in 4000 males. The defect can be caused by any one of hundreds of reported mutations but are most commonly due to inversions within intron 22 of the F.VIII gene. Hemophilia A has also been reported in a variety of species including dog and mouse, with a clinical phenotype very similar to human. The genetic defect in one case of canine Hemophilia-A has been shown to also be due to a gene inversion similar to the human defect, possibly indicating a common instability of the F.VIII gene in humans and dogs.

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