Candida albicans antigen ELISA Kit

Sample

serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Candida albicans antigen ELISA Kit is a quantitative and qualitative immunoassay for the detection of Candida antigen in human serum or plasma. The assay is recommended for the detection of systemic candidosis.

Contents of Kit

1. Break apart microtiter test strips each with eight antibody coated single wells,(altogether 96) MTP, 1 frame, 12 pieces.
2. Standard serum STD, 3×3 ml.
3. Negative control serum NEG, 2×3 ml.
4. Anti-Candida albicans conjugate (ready-to-use) PODCH, 13 ml.
5. Washing solution concentrate (sufficient for 1500 ml) WASH, 2×25 ml.
6. Dilution buffer SAMB, 15 ml.
7. Stopping solution STOP, 13 ml.
8. TMB-substrate solution (ready-to-use) TMB, 13 ml.
9. Quality control certificate with standard curve and evaluation table INFO, 2 pieces.

Storage

This kit should be stored at 2-8 °C.

Detection Limit

The limits of quantification are specified on the quality control certificate of the ELISA antigen test. The linearity of dilution within this range has been demonstrated in comprehensive evaluation studies. If a test sample shows a test result above the upper limit of quantification, the sample may be tested at a higher dilution, and the determined antigen quantity must be multiplied by this additional dilution factor.

General Description

Candida albicans is a ubiquitous yeast that belongs to the family of yeast-like fungi. Apart from the yeast form which primarily causes superficial infections, so called pseudomycelia are a further morphologic manifestation of yeast-like fungi. Germ tubes and the development of pseudo mycelia mainly occur in cases of systemic mycosis. Candida yeast-like fungi produce and excrete a range of destructive enzymes that enable this organism to penetrate blood vessels and mucosa barriers.
Candida ssp. are primarily transmitted by smear contamination from person to person. The primary portal of entry is the oral cavity. Changes in the fungistatic properties of the skin, which are a consequence of a slightly acidic pH and the antagonistic bacterial flora, can facilitate the establishment of superficial candidiasis of the skin surface. Systemic mycosis results from colonization of mucus membranes, particularly in the gastrointestinal tract. Candida spp. are able to adhere to the epithelia of mucosal membranes by adherence proteins and other cell surface structures.
Candida ssp. are not part of the physiological microflora of healthy humans. However, repeated colonization of the human host is frequently observed.