cAMP ELISA Kit (For Acetylated and Non-acetylated cAMP)

Name: cAMP ELISA Kit (For Acetylated and Non-acetylated cAMP)
SKU: DEIA-CA01
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Urine, plasma, culture medium samples

Species Reactivity

Universal

Detection Method

cELISA

Intended Use

The cAMP assay is a competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, sensitivity (80% B/B₀) of 0.1 pmol/ml for acetylated cAMP. Non-acetylated cAMP has a range from 0.3-750 pmol/ml and a sensitivity(80%B/B₀) of approximately 3 pmol/ml.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Cyclic AMP ELISA Anti serum	1vial/100 dtn	-20°C
2	Cyclic AMP AChE Tracer	1vial/100 dtn	-20°C
3	Cyclic AMP ELISA Standard	1 vial	-20°C
4	ELISA Buffer Concentrate(10×)	2vials/10 ml	-20°C
5	Wash Buffer Concentrate(400×)	1 vial/5 ml	-20°C
6	Polysorbate 20	1 vial/3 ml	-20°C
7	Mouse Anti-Rabbit lgG Coated Plate	1 plate	-20°C
8	96-Well Cover Sheet	1cover	-20°C
9	Ellman's Reagent	3 vials/100 dtn	-20°C
10	Acetic Anhydride	1 vial/2.5 ml	-20°C
11	Potassium Hydroxide	1 vial	-20°C
12	ELISA Tracer Dye	1vial	-20°C
13	ELISA Antiserum Dye	1 vial	-20°C

Storage

This kit will perform as specified if stored as directed at -20°C and used before the expiration date indicated on the outside of the box.

Precision

Non-Acetylated
The intra- and inter-assay CVs have been determined at multiple points on the standard curve.

Table. Intra- and inter-assay variation of the non-acetylated cAMP assay. *%CV represents the variation in concentration (not absorbance) as determined using a reference standard curve.
Acetylated
The intra- and inter-assay CVs have been determined at multiple points on the standard curve.

Table. Intra- and inter-assay variation of the acetylated cAMP assay.
*%CV represents the variation in concentration (not absorbance) as determined using a reference standard curve.

General Description

Adenosine 3',5' cyclic monophosphate (cAMP) is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of ligands or hormones to their specific G protein-coupled receptors activates GTP binding proteins (G_s or G_i) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.

Standard Curve

Sample Data (Non-Acetylated)